Can quaternary ammonium methacrylates inhibit matrix MMPs and cathepsins?

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dc.contributor.author Tezvergil-Mutluay, Arzu
dc.contributor.author Agee, Kelli A.
dc.contributor.author Mazzoni, Annalisa
dc.contributor.author Carvalho, Ricardo M.
dc.contributor.author Carrilho, Marcela
dc.contributor.author Tersariol, Ivarne L. [UNIFESP]
dc.contributor.author Nascimento, Fabio D.
dc.contributor.author Imazato, Satoshi
dc.contributor.author Tjaderhane, Leo
dc.contributor.author Breschi, Lorenzo
dc.contributor.author Tay, Franklin R.
dc.contributor.author Pashley, David H.
dc.date.accessioned 2016-01-24T14:39:59Z
dc.date.available 2016-01-24T14:39:59Z
dc.date.issued 2015-02-01
dc.identifier http://dx.doi.org/10.1016/j.dental.2014.10.006
dc.identifier.citation Dental Materials. Oxford: Elsevier B.V., v. 31, n. 2, p. E25-E32, 2015.
dc.identifier.issn 0109-5641
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38693
dc.description.abstract Objective. Dentin matrices release ICTP and CTX fragments during collagen degradation. ICTP fragments are known to be produced by MMPs. CTX fragments are thought to come from cathepsin K activity. the purpose of this study was to determine if quaternary methacrylates (QAMs) can inhibit matrix MMPs and cathepsins.Methods. Dentin beams were demineralizated, and dried to constant weight. Beams were incubated with rh-cathepsin B, K, L or S for 24 h at pH 7.4 to identify which cathepsins release CTX at neutral pH. Beams were dipped in ATA, an antimicrobial QAM to determine if it can inhibit dentin matrix proteases. Other beams were dipped in another QAM (MDPB) to determine if it produced similar inhibition of dentin proteases.Results. Only beams incubated with cathepsin K lost more dry mass than the controls and released CTX. Dentin beams dipped in ATA and incubated for 1 week at pH 7.4, showed a concentration-dependent reduction in weight-loss. There was no change in ICTP release from control values, meaning that ATA did not inhibit MMPs. Media concentrations of CTX fell significantly at 15 wt% ATA indicating that ATA inhibits capthesins.Beams dipped in increasing concentrations of MDPB lost progressively less mass, showing that MDPB is a protease-inhibitor. ICTP released from controls or beams exposed to low concentrations were the same, while 5 or 10% MDPB significantly lowered ICTP production. CTX levels were strongly inhibited by 2.5-10% MDPB, indicating that MDPB is a potent inhibitor of both MMPs and cathepsin K.Significance. CTX seems to be released from dentin matrix only by cathepsin K. MMPs and cathepsin K and B may all contribute to matrix degradation. (C) 2014 Academy of Dental Materials. Published by Elsevier B.V. All rights reserved. en
dc.description.sponsorship NIDCR
dc.description.sponsorship Academy of Finland
dc.description.sponsorship Finnish Dental Society Apollonia
dc.format.extent E25-E32
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Dental Materials
dc.rights Acesso restrito
dc.subject Degradation of collagen en
dc.subject CTX en
dc.subject ICTP en
dc.subject Quaternary ammonium compounds en
dc.subject MMPs en
dc.subject Cathepsins en
dc.title Can quaternary ammonium methacrylates inhibit matrix MMPs and cathepsins? en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Univ Turku
dc.contributor.institution Georgia Regents Univ
dc.contributor.institution Univ Bologna
dc.contributor.institution Univ British Columbia
dc.contributor.institution UNIBAN Univ Bandeirante Anhanguera
dc.contributor.institution Univ Mogi das Cruzes
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Osaka Univ
dc.contributor.institution Univ Oulu
dc.description.affiliation Univ Turku, Inst Dent, Adhes Dent Res Grp, Turku, Finland
dc.description.affiliation Georgia Regents Univ, Coll Dent Med, Dept Oral Biol, Augusta, GA 30912 USA
dc.description.affiliation Univ Bologna, Dept SAU& FAL, Bologna, Italy
dc.description.affiliation Univ British Columbia, Sch Dent, Dept Oral Sci, Vancouver, BC V5Z 1M9, Canada
dc.description.affiliation UNIBAN Univ Bandeirante Anhanguera, Biomat Res Grp, São Paulo, Brazil
dc.description.affiliation Univ Mogi das Cruzes, Ctr Interdisciplinar Invest Bioquim, Mogi Das Cruzes, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biochem, São Paulo, SP, Brazil
dc.description.affiliation Osaka Univ, Grad Sch Dent, Dept Biomat Sci, Osaka, Japan
dc.description.affiliation Univ Oulu, Oulu Univ Hosp, Inst Dent, Oulu, Finland
dc.description.affiliation Univ Turku, Inst Dent, Turku, Finland
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biochem, São Paulo, SP, Brazil
dc.description.sponsorshipID NIDCR: R01 DE015306
dc.identifier.doi 10.1016/j.dental.2014.10.006
dc.description.source Web of Science
dc.identifier.wos WOS:000349759400003



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