Porous bioactive scaffolds: characterization and biological performance in a model of tibial bone defect in rats

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dc.contributor.author Kido, Hueliton Wilian
dc.contributor.author Tim, Carla Roberta
dc.contributor.author Bossini, Paulo Sergio [UNIFESP]
dc.contributor.author Parizotto, Nivaldo Antonio
dc.contributor.author Castro, Cynthia Aparecida de
dc.contributor.author Crovace, Murilo Camuri
dc.contributor.author Martins Rodrigues, Ana Candida
dc.contributor.author Zanotto, Edgar Dutra
dc.contributor.author Peitl Filho, Oscar
dc.contributor.author Anibal, Fernanda de Freitas
dc.contributor.author Muniz Renno, Ana Claudia [UNIFESP]
dc.date.accessioned 2016-01-24T14:39:58Z
dc.date.available 2016-01-24T14:39:58Z
dc.date.issued 2015-02-01
dc.identifier http://dx.doi.org/10.1007/s10856-015-5411-9
dc.identifier.citation Journal of Materials Science-materials in Medicine. Dordrecht: Springer, v. 26, n. 2, 13 p., 2015.
dc.identifier.issn 0957-4530
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38679
dc.description.abstract The aim of this study was to evaluate the effects of highly porous Biosilicate (R) scaffolds on bone healing in a tibial bone defect model in rats by means of histological evaluation (histopathological and immunohistochemistry analysis) of the bone callus and the systemic inflammatory response (immunoenzymatic assay). Eighty Wistar rats (12 weeks-old, weighing +/-300 g) were randomly divided into 2 groups (n = 10 per experimental group, per time point): control group and Biosilicate (R) group (BG). Each group was euthanized 3, 7, 14 and 21 days post-surgery. Histological findings revealed a similar inflammatory response in both experimental groups, 3 and 7 days post-surgery. During the experimental periods (3-21 days post-surgery), it was observed that the biomaterial degradation, mainly in the periphery region, provided the development of the newly formed bone into the scaffolds. Immunohistochemistry analysis demonstrated that the Biosilicate (R) scaffolds stimulated cyclooxygenase-2, vascular endothelial growth factor and runtrelated transcription factor 2 expression. Furthermore, in the immunoenzymatic assay, BG presented no difference in the level of tumor necrosis factor alpha in all experimental periods. Still, BG showed a higher level of interleukin 4 after 14 days post-implantation and a lower level of interleukin 10 in 21 days post-surgery. Our results demonstrated that Biosilicate (R) scaffolds can contribute for bone formation through a suitable architecture and by stimulating the synthesis of markers related to the bone repair. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 13
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof Journal of Materials Science-materials in Medicine
dc.rights Acesso restrito
dc.title Porous bioactive scaffolds: characterization and biological performance in a model of tibial bone defect in rats en
dc.type Artigo
dc.rights.license http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institution Universidade Federal de São Carlos (UFSCar)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Fed Univ Sao Carlos UFSCar, Dept Physiotherapy, Postgrad Program Biotechnol, Sao Carlos, SP, Brazil
dc.description.affiliation Fed Univ São Paulo UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.affiliation Fed Univ Sao Carlos UFSCar, Dept Physiol Sci, Sao Carlos, SP, Brazil
dc.description.affiliation Fed Univ Sao Carlos UFSCar, Dept Mat Engn, Vitreous Mat Lab LaMaV, Sao Carlos, SP, Brazil
dc.description.affiliation Fed Univ Sao Carlos UFSCar, Dept Morphol & Pathol, Sao Carlos, SP, Brazil
dc.description.affiliationUnifesp Fed Univ São Paulo UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.identifier.doi 10.1007/s10856-015-5411-9
dc.description.source Web of Science
dc.identifier.wos WOS:000349402400014



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