Porous bioactive scaffolds: characterization and biological performance in a model of tibial bone defect in rats

Porous bioactive scaffolds: characterization and biological performance in a model of tibial bone defect in rats

Autor Kido, Hueliton Wilian Google Scholar
Tim, Carla Roberta Google Scholar
Bossini, Paulo Sergio Autor UNIFESP Google Scholar
Parizotto, Nivaldo Antonio Google Scholar
Castro, Cynthia Aparecida de Google Scholar
Crovace, Murilo Camuri Google Scholar
Martins Rodrigues, Ana Candida Google Scholar
Zanotto, Edgar Dutra Google Scholar
Peitl Filho, Oscar Google Scholar
Anibal, Fernanda de Freitas Google Scholar
Muniz Renno, Ana Claudia Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Carlos (UFSCar)
Universidade Federal de São Paulo (UNIFESP)
Resumo The aim of this study was to evaluate the effects of highly porous Biosilicate (R) scaffolds on bone healing in a tibial bone defect model in rats by means of histological evaluation (histopathological and immunohistochemistry analysis) of the bone callus and the systemic inflammatory response (immunoenzymatic assay). Eighty Wistar rats (12 weeks-old, weighing +/-300 g) were randomly divided into 2 groups (n = 10 per experimental group, per time point): control group and Biosilicate (R) group (BG). Each group was euthanized 3, 7, 14 and 21 days post-surgery. Histological findings revealed a similar inflammatory response in both experimental groups, 3 and 7 days post-surgery. During the experimental periods (3-21 days post-surgery), it was observed that the biomaterial degradation, mainly in the periphery region, provided the development of the newly formed bone into the scaffolds. Immunohistochemistry analysis demonstrated that the Biosilicate (R) scaffolds stimulated cyclooxygenase-2, vascular endothelial growth factor and runtrelated transcription factor 2 expression. Furthermore, in the immunoenzymatic assay, BG presented no difference in the level of tumor necrosis factor alpha in all experimental periods. Still, BG showed a higher level of interleukin 4 after 14 days post-implantation and a lower level of interleukin 10 in 21 days post-surgery. Our results demonstrated that Biosilicate (R) scaffolds can contribute for bone formation through a suitable architecture and by stimulating the synthesis of markers related to the bone repair.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Data de publicação 2015-02-01
Publicado em Journal of Materials Science-materials in Medicine. Dordrecht: Springer, v. 26, n. 2, 13 p., 2015.
ISSN 0957-4530 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 13
Fonte http://dx.doi.org/10.1007/s10856-015-5411-9
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000349402400014
Endereço permanente http://repositorio.unifesp.br/handle/11600/38679

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta