Diversity of Shiga toxin-producing Escherichia coli in sheep flocks of Parana State, southern Brazil

Diversity of Shiga toxin-producing Escherichia coli in sheep flocks of Parana State, southern Brazil

Author Martins, Fernando Henrique Google Scholar
Cabilio Guth, Beatriz Ernestina Autor UNIFESP Google Scholar
Piazza, Roxane Maria Google Scholar
Leao, Sylvia Cardoso Autor UNIFESP Google Scholar
Ludovico, Agostinho Google Scholar
Ludovico, Marilucia Santos Google Scholar
Dahbi, Ghizlane Google Scholar
Marzoa, Juan Google Scholar
Mora, Azucena Google Scholar
Blanco, Jorge Google Scholar
Pelayo, Jacinta Sanchez Google Scholar
Institution Universidade Estadual de Londrina (UEL)
Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Univ Norte Parana
USC
Abstract Sheep constitute an important source of zoonotic pathogens as Shiga toxin-producing Escherichia coli (STEC). in this study, the prevalence, serotypes and virulence profiles of STEC were investigated among 130 healthy sheep from small and medium farms in southern Brazil. STEC was isolated from 65 (50%) of the tested animals and detected in all flocks. A total of 70 STEC isolates were characterized, and belonged to 23 different O:H serotypes, many of which associated with human disease, including hemolytic-uremic syndrome (HUS). Among the serotypes identified, O76:H19 and O65:H- were the most common, and O75:H14 and O169:H7 have not been previously reported in STEC strains. Most of the STEC isolates harbored only stx1, whereas the Stx2b subtype was the most common among those carrying stx2. Enterohemolysin (ehxA) and intimin (eae) genes were detected in 61 (87.1%) and four (5.7%) isolates, respectively. Genes encoding putative adhesins (saa, iha, lpf(O113)) and toxins (subAB and cdtV) were also observed. the majority of the isolates displayed virulence features related to pathogenesis of STEC, such as adherence to epithelial cells, high cytotoxicity and enterohemolytic activity. Ovine STEC isolates belonged mostly to phylogenetic group B1. PFGE revealed particular clones distributed in some farms, as well as variations in the degree of genetic similarity within serotypes examined. in conclusion, STEC are widely distributed in southern Brazilian sheep, and belonged mainly to serotypes that are not commonly reported in other regions, such as 076:H19 and 065:H-. A geographical variation in the distribution of STEC serotypes seems to occur in sheep. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Sheep
Shiga toxin-producing E. coli
Serotypes
Virulence factors
Genetic diversity
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Conselleria de Cultura, Educacion e Ordenacion Universitaria, Xunta de Galicia
European Regional Development Fund (ERDF)
Ministerio de Economia y Competitividad, Gobierno de Espana
Grant number European Regional Development Fund (ERDF): CN2012/303
Ministerio de Economia y Competitividad, Gobierno de Espana: AGL2013-47852-R
Date 2015-01-30
Published in Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 175, n. 1, p. 150-156, 2015.
ISSN 0378-1135 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 150-156
Origin http://dx.doi.org/10.1016/j.vetmic.2014.11.003
Access rights Closed access
Type Article
Web of Science ID WOS:000348083100020
URI http://repositorio.unifesp.br/handle/11600/38645

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