Homemade Capillary Electrophoresis Coupled to a Mass Spectrometer

Homemade Capillary Electrophoresis Coupled to a Mass Spectrometer

Author Moraes, Janderson Aparecido de Autor UNIFESP Google Scholar
Carrilho, Emanuel Google Scholar
Assunção, Nilson Antonio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract A system was developed in our laboratory to couple capillary electrophoresis with mass spectrometry. the capillary electrophoresis system was equipped with a high voltage supply, and a microcontroller with assembly language programming was developed for the computational control of the system. the MS system was a commercial Thermo Finningan LCQ ion trap mass spectrometer. the robustness of the coupled system was evaluated using standard protein samples (lysozyme, aprotinin, and bovine albumin) and tryptic digests of lysozyme. the system showed positive results in terms of robustness, allowing for the separation of digested proteins and the identification of 33% of the total amino acids in a protein (6 of the 18 expected peptides). the limit of detection was in the order of 1 picomole (signal-to-noise ratio), which was considered satisfactory for this system. the system shows high versatility in tandem coupling and combinations with other analytical procedures.
Keywords mass spectrometry
instrumentation
proteomics
capillary electrophoresis
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2015-01-02
Published in Journal of Liquid Chromatography & Related Technologies. Philadelphia: Taylor & Francis Inc, v. 38, n. 1, p. 36-43, 2015.
ISSN 1082-6076 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Inc
Extent 36-43
Origin http://dx.doi.org/10.1080/10826076.2013.864982
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000344381300006
URI http://repositorio.unifesp.br/handle/11600/38629

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