Serine proteases as candidates for proteolytic processing of angiotensin-I converting enzyme

Serine proteases as candidates for proteolytic processing of angiotensin-I converting enzyme

Author Aragao, Danielle S. Autor UNIFESP Google Scholar
Andrade, Maria Claudina C. de Google Scholar
Ebihara, Fabiana Autor UNIFESP Google Scholar
Watanabe, Ingrid K. M. Autor UNIFESP Google Scholar
Magalhaes, Dayane C. B. P. Autor UNIFESP Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Hirata, Izaura Yoshico Autor UNIFESP Google Scholar
Casarini, Dulce Elena Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Somatic angiotensin-I converting enzyme (sACE) is a broadly distributed peptidase which plays a role in blood pressure and electrolyte homeostasis by the conversion of angiotensin I into angiotensin II. N-domain isoforms (nACE) with 65 and 90 kDa have been described in body fluids, tissues and mesangial cells (MC), and a 90 kDa nACE has been described only in spontaneously hypertensive rats. the aim of this study was to investigate the existence of proteolytic enzymes that may act in the hydrolysis of sACE generating nACEs in MC. After the confirmation of the presence of ACE sheddases in Immortalized MC (IMC), we purified and characterized these enzymes using fluorogenic substrates specifically designed for ACE sheddases. Purified enzyme identified as a serine protease by N-terminal sequence was able to generate nACE. in the present study, we described for the first time the presence of ACE sheddases in IMC, identified as serine proteases able to hydrolyze sACE in vitro. Further investigations are necessary to elucidate the mechanisms responsible for the expression and regulation of ACE sheddases in MC and their roles in the generation of nACEs, especially the 90 kDa form possibly related to hypertension. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Angiotensin-I converting enzyme
Shedding, Mesangial cells
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2008/55771-3
FAPESP: 2010/51904-9
Date 2015-01-01
Published in International Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 72, p. 673-679, 2015.
ISSN 0141-8130 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 673-679
Access rights Closed access
Type Article
Web of Science ID WOS:000347577900087

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