Palmitoleic acid (n-7) increases white adipocytes GLUT4 content and glucose uptake in association with AMPK activation

Palmitoleic acid (n-7) increases white adipocytes GLUT4 content and glucose uptake in association with AMPK activation

Author Bolsoni-Lopes, Andressa Google Scholar
Festuccia, William T. Google Scholar
Chimin, Patricia Google Scholar
Farias, Talita S. M. Google Scholar
Torres-Leal, Francisco L. Google Scholar
Cruz, Maysa M. Autor UNIFESP Google Scholar
Andrade, Paula B. Google Scholar
Hirabara, Sandro M. Google Scholar
Lima, Fabio B. Google Scholar
Alonso-Vale, Maria Isabel C. Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Cruzeiro Univ
Abstract Background: Palmitoleic acid was previously shown to improve glucose homeostasis by reducing hepatic glucose production and by enhancing insulin-stimulated glucose uptake in skeletal muscle. Herein we tested the hypothesis that palmitoleic acid positively modulates glucose uptake and metabolism in adipocytes.Methods: for this, both differentiated 3 T3-L1 cells treated with either palmitoleic acid (16: 1n7, 200 mu M) or palmitic acid (16: 0, 200 mu M) for 24 h and primary adipocytes from mice treated with 16: 1n7 (300 mg/kg/day) or oleic acid (18: 1n9, 300 mg/kg/day) by gavage for 10 days were evaluated for glucose uptake, oxidation, conversion to lactate and incorporation into fatty acids and glycerol components of TAG along with the activity and expression of lipogenic enzymes.Results: Treatment of adipocytes with palmitoleic, but not oleic (in vivo) or palmitic (in vitro) acids, increased basal and insulin-stimulated glucose uptake and GLUT4 mRNA levels and protein content. Along with uptake, palmitoleic acid enhanced glucose oxidation (aerobic glycolysis), conversion to lactate (anaerobic glycolysis) and incorporation into glycerol-TAG, but reduced de novo fatty acid synthesis from glucose and acetate and the activity of lipogenic enzymes glucose 6-phosphate dehydrogenase and ATP-citrate lyase. Importantly, palmitoleic acid induction of adipocyte glucose uptake and metabolism were associated with AMPK activation as evidenced by the increased protein content of phospho(p) Thr172AMPKa, but no changes in pSer473Akt and pThr308Akt. Importantly, such increase in GLUT4 content induced by 16: 1n7, was prevented by pharmacological inhibition of AMPK with compound C.Conclusions: in conclusion, palmitoleic acid increases glucose uptake and the GLUT4 content in association with AMPK activation.
Keywords GLUT4
de novo lipogenesis
Glucose oxidation
Glucose metabolism
Language English
Date 2014-12-20
Published in Lipids in Health and Disease. London: Biomed Central Ltd, v. 13, 10 p., 2014.
ISSN 1476-511X (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 10
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000348818200001

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