Dynein Function and Protein Clearance Changes in Tumor Cells Induced by a Kunitz-Type Molecule, Amblyomin-X

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dc.contributor.author Pacheco, Mario T. F.
dc.contributor.author Berra, Carolina M.
dc.contributor.author Morais, Katia Luciano Pereira [UNIFESP]
dc.contributor.author Sciani, Juliana M.
dc.contributor.author Branco, Vania G.
dc.contributor.author Bosch, Rosemary V.
dc.contributor.author Chudzinski-Tavassi, Ana M.
dc.date.accessioned 2016-01-24T14:38:18Z
dc.date.available 2016-01-24T14:38:18Z
dc.date.issued 2014-12-05
dc.identifier http://dx.doi.org/10.1371/journal.pone.0111907
dc.identifier.citation Plos One. San Francisco: Public Library Science, v. 9, n. 12, 20 p., 2014.
dc.identifier.issn 1932-6203
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38543
dc.description.abstract Amblyomin-X is a Kunitz-type recombinant protein identified from the transcriptome of the salivary glands of the tick Amblyomma cajennense and has anti-coagulant and antitumoral activity. the supposed primary target of this molecule is the proteasome system. Herein, we elucidated intracellular events that are triggered by Amblyomin-X treatment in an attempt to provide new insight into how this serine protease inhibitor, acting on the proteasome, could be comparable with known proteasome inhibitors. the collective results showed aggresome formation after proteasome inhibition that appeared to occur via the non-exclusive ubiquitin pathway. Additionally, Amblyomin-X increased the expression of various chains of the molecular motor dynein in tumor cells, modulated specific ubiquitin linkage signaling and inhibited autophagy activation by modulating mTOR, LC3 and AMBRA1 with probable dynein involvement. Interestingly, one possible role for dynein in the mechanism of action of Amblyomin-X was in the apoptotic response and its crosstalk with autophagy, which involved the factor Bim; however, we observed no changes in the apoptotic response related to dynein in the experiments performed. the characteristics shared among Amblyomin-X and known proteasome inhibitors included NF-kappa B blockage and nascent polypeptide-dependent aggresome formation. Therefore, our study describes a Kunitz-type protein that acts on the proteasome to trigger distinct intracellular events compared to classic known proteasome inhibitors that are small-cell-permeable molecules. in investigating the experiments and literature on Amblyomin-X and the known proteasome inhibitors, we also found differences in the structures of the molecules, intracellular events, dynein involvement and tumor cell type effects. These findings also reveal a possible new target for Amblyomin-X, i.e., dynein, and may serve as a tool for investigating tumor cell death associated with proteasome inhibition. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 20
dc.language.iso eng
dc.publisher Public Library Science
dc.relation.ispartof Plos One
dc.rights Acesso aberto
dc.title Dynein Function and Protein Clearance Changes in Tumor Cells Induced by a Kunitz-Type Molecule, Amblyomin-X en
dc.type Artigo
dc.contributor.institution Butantan Inst
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Butantan Inst, Biochem & Biophys Lab, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biochem, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2011/05969-4
dc.description.sponsorshipID FAPESP: CAT/CEPID 1998/14307-9
dc.description.sponsorshipID FAPESP: CETICs 2013/07467-1
dc.identifier.file WOS000346907200006.pdf
dc.identifier.doi 10.1371/journal.pone.0111907
dc.description.source Web of Science
dc.identifier.wos WOS:000346907200006


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