The beneficial effects of strength exercise on hippocampal cell proliferation and apoptotic signaling is impaired by anabolic androgenic steroids

The beneficial effects of strength exercise on hippocampal cell proliferation and apoptotic signaling is impaired by anabolic androgenic steroids

Author Novaes Gomes, Fabiano Guimaraes Autor UNIFESP Google Scholar
Fernandes, Jansen Autor UNIFESP Google Scholar
Campos, Diego Vannucci Autor UNIFESP Google Scholar
Cassilhas, Ricardo Cardoso Autor UNIFESP Google Scholar
Viana, Gustavo Monteiro Autor UNIFESP Google Scholar
D'Almeida, Vania Autor UNIFESP Google Scholar
Moraes Rego, Marta Karavisch de Autor UNIFESP Google Scholar
Buainain, Pedro Ivo Autor UNIFESP Google Scholar
Cavalheiro, Esper Abrao Autor UNIFESP Google Scholar
Arida, Ricardo Mario Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fac Minas FAMINAS
Abstract Previous studies have shown that strength exercise improves memory and increases expression of a myriad of proteins involved on neuronal survival and synaptic plasticity in the hippocampus. Conversely, chronic exposure to supraphysiological levels of anabolic androgenic steroids (AAS) can induce psychiatric abnormalities, cognitive deficits, impair neurotransmission, alter the levels of neurotrophic factors, decrease cell proliferation and neurogenesis, and enhance neuronal cell death. in the present study, we investigated the effects of the MS nandrolone decanoate (ND) administration during a strength exercise program on cell proliferation, apoptotic status and brain-derived neurotrophic factor (BDNF) expression in the rat hippocampus. Adult male Wistar rats were subjected to 4 weeks of progressive strength exercise in a vertical ladder apparatus with or without daily doses (5.0 mg/kg, SC) of ND. Immunohistochemistry analysis revealed that strength exercise increased significantly the number of Ki-67-positive cells (a cell proliferation marker) in dentate gyrus (DG) of hippocampus. However, this effect was abrogated when strength exercise was combined with ND. Although western blot analysis of whole hippocampus showed no significant differences in Bax and Bcl-2 protein expression among groups, the immunoreactivity of the pro-apoptotic protein Bax was significantly increased in DG, CA1 and CA3 hippocampal subfields of sedentary rats treated with ND. Moreover, the increase in the immunoreactivity of anti-apoptotic protein Bcl-2 (DG and CA3) induced by strength exercise was diminished by ND. There were no significant differences in BDNF expression among experimental groups. Therefore, the present findings suggest that the beneficial effects of strength exercise on hippocampal cell proliferation and apoptotic signaling are impaired by ND. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Anabolic androgenic steroids
Strength exercise
Hippocampus
Apoptosis
BDNF
Neuroplasticity
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Instituto Nacional de Neurociencia Translacional (INNT)
Date 2014-12-01
Published in Psychoneuroendocrinology. Oxford: Pergamon-Elsevier B.V., v. 50, p. 106-117, 2014.
ISSN 0306-4530 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 106-117
Origin http://dx.doi.org/10.1016/j.psyneuen.2014.08.009
Access rights Closed access
Type Article
Web of Science ID WOS:000345061000010
URI http://repositorio.unifesp.br/handle/11600/38517

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