Characterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatus

Characterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatus

Author Ortega, P. Google Scholar
Custodio, M. R. Google Scholar
Zanotto, F. P. Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Membrane pathway for intracellular cadmium (Cd2+) accumulation is not fully elucidated in many organisms and has not been studied in crab gill cells. To characterize membrane Cd2+ transport of anterior and posterior gill cells of Ucides cordatus, a hypo-hyper-regulating crab, a change in intracellular Cd2+ concentration under various experimental conditions was examined by using FluoZin, a fluorescent probe. the membrane Cd2+ transport was estimated by the augmentation of FluoZin fluorescence induced by extracellular application of CdCl2 and different inhibitors. Addition of extracellular calcium (Cd2+) to the cells affected little the fluorescence of FluoZin, confirming that Cd2+ was the main ion increasing intracellular fluorescence. Cd2+ channels blockers (nimodipine and verapamil) decreased Cd2+ influx as well as vanadate, a Cd2+-ATPase blocker. Chelating intracellular Cd2+ (BAPTA) decreased Cd2+ influx in gill cells, while increasing intracellular Cd2+ (caffeine) augmented Cd influx. Cd2+ and ATP added at different temporal conditions were not effective at increasing intracellular Cd2+ accumulation. Ouabain (Na+/K+-ATPase inhibitor) increased Cd2+ influx probably through a change in intracellular Na and/or a change in cell membrane potential. Routes of Cd2+ influx, a non-essential metal, through the gill cell plasma membrane of crabs are suggested. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Ucides cordatus
Cadmium transport
Gill cells
Calcium channel blockers
Cell transport inhibitors
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2009/15546-3
Date 2014-12-01
Published in Aquatic Toxicology. Amsterdam: Elsevier B.V., v. 157, p. 21-29, 2014.
ISSN 0166-445X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 21-29
Access rights Closed access
Type Article
Web of Science ID WOS:000346547800003

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