Reaction time and replenishment time of SP and CGRP after incision in rat skin

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dc.contributor.author Hochman, Bernardo [UNIFESP]
dc.contributor.author Isoldi, Felipe Contoli [UNIFESP]
dc.contributor.author Freitas, Jose Octavio Gonçalves de [UNIFESP]
dc.contributor.author Lapin, Guilherme Abbud Franco [UNIFESP]
dc.contributor.author Espírito Santo, Paulo Rogério Quieregatto do [UNIFESP]
dc.contributor.author Silva, Erica Calcagno Raymundo da [UNIFESP]
dc.contributor.author Chadi, Gerson
dc.contributor.author Ferreira, Lydia Masako [UNIFESP]
dc.date.accessioned 2016-01-24T14:38:14Z
dc.date.available 2016-01-24T14:38:14Z
dc.date.issued 2014-12-01
dc.identifier http://dx.doi.org/10.2478/s11535-014-0349-3
dc.identifier.citation Central European Journal of Biology. Warsaw: de Gruyter Open Ltd, v. 9, n. 12, p. 1140-1146, 2014.
dc.identifier.issn 1895-104X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38490
dc.description.abstract Background. the skin neurogenic inflammation is mainly related to Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). There is no data on their availability in the dynamics of skin nerve endings, concerning their release and replenishment after a nociceptive stimulus, so this was investigated. Materials and methods. 25 rats were randomly distributed in 5 groups. the animals of the control group (CG) determined the baseline levels of neuropeptides in the skin. the groups S0 and S30 did not receive any cutaneous stimulus at 30 and 60 minutes, respectively. in the group S1, an incision stimulus was made at 30 minutes. in the group S31, a nociceptive stimulus was performed by subdermal scratching at 30 minutes and, at 60 minutes, the incision stimulus was carried out in the same location (nociceptive hyperstimulation). the skin samples of the other animals were harvested from the back 1 minute after their death. SP, pro-CGRP and CGRP were quantified by Western Blotting. Results. the incision stimulus released SP, S1 compared to S0 (p < 0.05) detected in the first minute, and the replenishment time was more than 30 minutes. Also, it cleaved pro-CGRP, S1 compared to S31 (p < 0.05) in the first minute, and its replenishment time less than 30 minutes. Release of CGRP was not detected. Conclusion. the incision released SP already detected in the first minute; its replenishment time is more than 30 minutes. the incision decreased pro-CGRP, also detected in the first minute; and its replenishment time is less than 30 minutes. en
dc.format.extent 1140-1146
dc.language.iso eng
dc.publisher De Gruyter Open Ltd
dc.relation.ispartof Central European Journal of Biology
dc.rights Acesso aberto
dc.subject SP en
dc.subject CGRP en
dc.subject Neurogenic Inflammation en
dc.subject Neuropeptides en
dc.subject Reaction Time en
dc.subject Temporal Distribution en
dc.title Reaction time and replenishment time of SP and CGRP after incision in rat skin en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Fed Univ São Paulo Unifesp, Dept Surg, Div Plast Surg, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Neurol, São Paulo, Brazil
dc.description.affiliationUnifesp Fed Univ São Paulo Unifesp, Dept Surg, Div Plast Surg, São Paulo, Brazil
dc.identifier.file WOS000340681200002.pdf
dc.identifier.doi 10.2478/s11535-014-0349-3
dc.description.source Web of Science
dc.identifier.wos WOS:000340681200002



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