Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves

Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves

Author Casagrande, Junior Cesar Google Scholar
Benitez Macorini, Luis Fernando Google Scholar
Antunes, Katia Avila Google Scholar
Santos, Uilson Pereira dos Google Scholar
Campos, Jaqueline Ferreira Google Scholar
Dias-Junior, Nelson Miguel Google Scholar
Sangalli, Andreia Google Scholar
Lima Cardoso, Claudia Andrea Google Scholar
Vieira, Maria do Carmo Google Scholar
Rabelo, Luiza Antas Google Scholar
Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Santos, Edson Lucas dos Google Scholar
Souza, Kely de Picoli Google Scholar
Institution Fed Univ Grande Dourados
Universidade Estadual de Mato Grosso do Sul (UEMS)
Univ Fed Alagoas
Universidade Federal de São Paulo (UNIFESP)
Abstract Background and Purpose: Leaves of Jacaranda decurrens are used in traditional Brazilian medicine to treat metabolic diseases related to increased reactive oxygen species. the present study evaluated the antioxidant and cytotoxic potential of hydroethanolic extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata.Experimental Approach: Phenolic compounds, flavonoids and saponins were evaluated in an ethanol: water (80: 20, v/v) extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata (E-Jds). the antioxidant activity of E-Jds was investigated by assessing the following: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity; protection against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis of erythrocytes; in vitro and in vivo malondialdehyde dosage; and the ability to activate antioxidant enzymes. K562 leukemia cells were used for the cytotoxic evaluation of E-Jds and for the assessment of the cell death profile through flow cytometry.Key Results: Phenolic and flavonoid compounds were quantified as 14.38% and 2.15%, respectively, of E-Jds. These phenolic and flavonoid compounds proved to be able to scavenge DPPH free radicals with an IC50 of 9.3 +/- 3.3 mu g/mL, to protect up to 50% of erythrocytes against AAPH-induced hemolysis and to reduce in vitro and in vivo malondialdehyde levels up to 84% and 22%, respectively. E-Jds also increased glutathione peroxidase enzyme activity, with a concomitant decrease in superoxide dismutase and catalase activity, and exhibited dose-dependent cytotoxic activity on K562 erythroleukemia cells with cell death occurring via both late apoptosis and necrosis.Conclusions: E-Jds exhibits in vitro and in vivo antioxidant potential, which may be the mechanism mediating the metabolic activities reported in folk medicine. Furthermore, the cytotoxic activity identified in this study contributes with the knowledge of antiproliferative activities that have been described in the literature for the genus Jacaranda.
Language English
Sponsor Fundacao de Apoio ao Desenvolvimento do Ensino
Ciencia e Tecnologia do Mato Grosso do Sul (FUNDECT)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-11-17
Published in Plos One. San Francisco: Public Library Science, v. 9, n. 11, 8 p., 2014.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 8
Origin http://dx.doi.org/10.1371/journal.pone.0112748
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000345158700067
URI http://repositorio.unifesp.br/handle/11600/38458

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