A sensitive and specific molecular tool for detection of both typical and atypical enteroaggregative Escherichia coli

A sensitive and specific molecular tool for detection of both typical and atypical enteroaggregative Escherichia coli

Author Andrade, Fernanda B. Google Scholar
Gomes, Tania A. T. Autor UNIFESP Google Scholar
Elias Junior, Waldir Pereira Autor UNIFESP Google Scholar
Institution Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Abstract A multiplex PCR has been standardized and evaluated for the identification of both typical and atypical enteroaggregative Escherichia coli (EAEC). the assay detecting aaiA, aaiG, aggR and aatA presented 94.8% of sensitivity, 94.3% of specificity and was able to efficiently detect both groups of EAEC among E. coli isolated from stool cultures. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Enteroaggregative Escherichia coli
EAEC
Multiplex PCR
Sensitivity
Specificity
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2010/19515-2
Date 2014-11-01
Published in Journal of Microbiological Methods. Amsterdam: Elsevier B.V., v. 106, p. 16-18, 2014.
ISSN 0167-7012 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 16-18
Origin http://dx.doi.org/10.1016/j.mimet.2014.07.030
Access rights Closed access
Type Article
Web of Science ID WOS:000343358200003
URI http://repositorio.unifesp.br/handle/11600/38403

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