A simple and effective method to synthesize fluorescent nanoparticles using tryptophan and light and their lethal effect against bacteria

A simple and effective method to synthesize fluorescent nanoparticles using tryptophan and light and their lethal effect against bacteria

Author Tomita, Rafael Jun Autor UNIFESP Google Scholar
Matos, Ricardo Almeida de Autor UNIFESP Google Scholar
Vallim, Marcelo Afonso Autor UNIFESP Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract A simple, environmentally friendly and cost-effective method was used to synthesize silver nanoparticles using typtophan and light. To prepare the nanoparticles, the following components were used: deionized water, silver nitrate, light and tryptophan. the effects of the tryptophan concentration and light exposure time on the formation of tryptophan silver nanoparticles (Tnnps) were studied. the synthesized Tnnps were characterized using transmission electron microscopy (TEM), absorption and fluorescence spectroscopy and zeta potential measurements. the synthesized Tnnps were nearly spherical, with sizes of approximately 17 nm. in addition, the antibacterial activity of Tnnps was determined by monitoring the growth curves of strains of Escherichia colt, Pseudomonas aeruginosa, Staphylococcus epidermidis, Serratia marcescens, and Enterococcus faecalis using the microdilution test. the Minimum Inhibitory Concentration (MIC) for 4 of 5 tested bacteria was determined to be between 20.0 and 17.5 mu g/mL for 48 h and between 22.5 and 20.0 mu g/mL for 72 h. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Tryptophan
Silver nanoparticles
Photoreduction
MIC
Bacteria
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2014-11-01
Published in Journal of Photochemistry and Photobiology B-biology. Lausanne: Elsevier Science Sa, v. 140, p. 157-162, 2014.
ISSN 1011-1344 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 157-162
Origin http://dx.doi.org/10.1016/j.jphotobiol.2014.07.015
Access rights Closed access
Type Article
Web of Science ID WOS:000347018600020
URI http://repositorio.unifesp.br/handle/11600/38400

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