Evaluation of glycosaminoglycans and heparanase in placentas of women with preeclampsia

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dc.contributor.author Brosco Fama, Eduardo Augusto
dc.contributor.author Souza, Renan Salvioni
dc.contributor.author Melo, Carina Mucciolo [UNIFESP]
dc.contributor.author Pompei, Luciano Melo
dc.contributor.author Silva Pinhal, Maria Aparecida [UNIFESP]
dc.date.accessioned 2016-01-24T14:38:05Z
dc.date.available 2016-01-24T14:38:05Z
dc.date.issued 2014-11-01
dc.identifier http://dx.doi.org/10.1016/j.cca.2014.07.023
dc.identifier.citation Clinica Chimica Acta. Amsterdam: Elsevier B.V., v. 437, p. 155-160, 2014.
dc.identifier.issn 0009-8981
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38388
dc.description.abstract Background: Preedampsia is a multisystem disorder whose etiology remains unclear. It is already known that circulation of soluble fms-like tyrosine kinase-1 (sFlt-1) is directly involved in pre-eclampsia development. However, the molecular mechanisms involved with sFlt-1 shedding are still unidentified. We identified, quantified glycosaminoglycans and determined the enzymatic activity of heparanase in placentas of women with preeclampsia, in order to possibly explain if these compounds could be related to cellular processes involved with preeclampsia.Methods:A total of 45 samples collected from placentas, 15 samples from placentas of preeclampsia women and 30 samples from non-affected women. Heparan sulfate and dermatan sulfate were identified and quantified by agarose gel electrophoresis, whilst hyaluronic acid was quantified by an ELISA like assay. Heparanase activity was determined using biotynilated heparan sulfate as substrate.Results: the results showed that dermatan sulfate (P = 0.019), heparan sulfate levels (P = 0.015) and heparanase activity (P = 0.006) in preeclampsia were significantly higher than in the control group. There was no significant difference between the groups for hyaluronic acid expression in placentas (P = 0.110). the present study is the first to demonstrate directly the increase of heparan sulfate in human placentas from patients with preeclampsia, suggesting that endogenous heparan sulfate could be involved in the release of sFlt-1 from placenta, increasing the level of circulating sFlt-1.Conclusion: Alterations of extracellular matrix components in placentas with preeclampsia raise the possibility that heparan sulfate released by heparanase is involved in mechanisms of preeclampsia development. Published by Elsevier B.V. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 155-160
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Clinica Chimica Acta
dc.rights Acesso restrito
dc.subject Preeclampsia en
dc.subject Heparan sulfate en
dc.subject Dermatan sulfate en
dc.subject Hyaluronic acid en
dc.subject Heparanase en
dc.title Evaluation of glycosaminoglycans and heparanase in placentas of women with preeclampsia en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution FMABC
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation FMABC, Obstet Gynecol Dept, São Paulo, Brazil
dc.description.affiliation FMABC, Dept Biochem, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo UNIFESP, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP, Dept Biochem, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2009/50061-0
dc.identifier.doi 10.1016/j.cca.2014.07.023
dc.description.source Web of Science
dc.identifier.wos WOS:000342246900027



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