Evaluation of glycosaminoglycans and heparanase in placentas of women with preeclampsia

Evaluation of glycosaminoglycans and heparanase in placentas of women with preeclampsia

Author Brosco Fama, Eduardo Augusto Google Scholar
Souza, Renan Salvioni Google Scholar
Melo, Carina Mucciolo Autor UNIFESP Google Scholar
Pompei, Luciano Melo Google Scholar
Silva Pinhal, Maria Aparecida Autor UNIFESP Google Scholar
Institution FMABC
Universidade Federal de São Paulo (UNIFESP)
Abstract Background: Preedampsia is a multisystem disorder whose etiology remains unclear. It is already known that circulation of soluble fms-like tyrosine kinase-1 (sFlt-1) is directly involved in pre-eclampsia development. However, the molecular mechanisms involved with sFlt-1 shedding are still unidentified. We identified, quantified glycosaminoglycans and determined the enzymatic activity of heparanase in placentas of women with preeclampsia, in order to possibly explain if these compounds could be related to cellular processes involved with preeclampsia.Methods:A total of 45 samples collected from placentas, 15 samples from placentas of preeclampsia women and 30 samples from non-affected women. Heparan sulfate and dermatan sulfate were identified and quantified by agarose gel electrophoresis, whilst hyaluronic acid was quantified by an ELISA like assay. Heparanase activity was determined using biotynilated heparan sulfate as substrate.Results: the results showed that dermatan sulfate (P = 0.019), heparan sulfate levels (P = 0.015) and heparanase activity (P = 0.006) in preeclampsia were significantly higher than in the control group. There was no significant difference between the groups for hyaluronic acid expression in placentas (P = 0.110). the present study is the first to demonstrate directly the increase of heparan sulfate in human placentas from patients with preeclampsia, suggesting that endogenous heparan sulfate could be involved in the release of sFlt-1 from placenta, increasing the level of circulating sFlt-1.Conclusion: Alterations of extracellular matrix components in placentas with preeclampsia raise the possibility that heparan sulfate released by heparanase is involved in mechanisms of preeclampsia development. Published by Elsevier B.V.
Keywords Preeclampsia
Heparan sulfate
Dermatan sulfate
Hyaluronic acid
Heparanase
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2009/50061-0
Date 2014-11-01
Published in Clinica Chimica Acta. Amsterdam: Elsevier B.V., v. 437, p. 155-160, 2014.
ISSN 0009-8981 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 155-160
Origin http://dx.doi.org/10.1016/j.cca.2014.07.023
Access rights Closed access
Type Article
Web of Science ID WOS:000342246900027
URI http://repositorio.unifesp.br/handle/11600/38388

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