Rmcystatin3, a cysteine protease inhibitor from Rhipicephalus microplus hemocytes involved in immune response

Rmcystatin3, a cysteine protease inhibitor from Rhipicephalus microplus hemocytes involved in immune response

Author Lu, Stephen Autor UNIFESP Google Scholar
Soares, Tatiane Sanches Autor UNIFESP Google Scholar
Vaz Junior, Itabajara S. Google Scholar
Lovato, Diogo Ventura Autor UNIFESP Google Scholar
Tanaka, Aparecida Sadae Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande do Sul
Abstract The Rhipicephalus microplus tick is responsible for losses in the livestock production estimated in 2 billions USD. Despite its economical importance the knowledge in tick's physiology is sparse. in order to contribute to this scenario we describe the characterization of a cysteine proteinase inhibitor named Rmcystatin-3. Purified recombinant Rmcystatin-3 was able to inhibit cathepsin L (Ki = 2.5 nM), BmCl1 (Ki = 1.8 nM) and cathepsin B (Ki = 136 nM). Western blot and quantitative PCR analysis revealed the presence of Rmcystatin-3 in fat body, salivary gland but mainly in hemocytes. the mRNA levels of Rmcystatin-3 during bacterial challenge are drastically down-regulated. in order to define the Rmcystatin-3 possible role in tick immunity, the cystatin gene was knockdown by RNA interference with and without Escherichia coli infection. Our results showed that the Rmcystatin-3 silenced group was more immune competent to control bacterial infection than the group injected with non-related dsRNA. Taking together, our data strongly suggested an important role of Rmcystatin-3 in tick immunity. (C) 2014 Elsevier B.V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved.
Keywords Cystatin
RNAi
Rhipicephalus microplus
Ticks
Innate immunity
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
INCT-Entomologia Molecular, Brazil
Grant number FAPESP: 05/03514-9
FAPESP: 09/17589-1
FAPESP: 12/03657-8
Date 2014-11-01
Published in Biochimie. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 106C, p. 17-23, 2014.
ISSN 0300-9084 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 17-23
Origin http://dx.doi.org/10.1016/j.biochi.2014.07.012
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000344434700003
URI http://repositorio.unifesp.br/handle/11600/38351

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