TGF-beta(1) expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding

TGF-beta(1) expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding

Author Alves, Claudia Cristina Google Scholar
Torrinhas, Raquel Susana Google Scholar
Giorgi, Ricardo Google Scholar
Brentani, Maria Mitzi Google Scholar
Logullo, Angela Flavia Autor UNIFESP Google Scholar
Waitzberg, Dan Linetzky Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Malnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post-trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT-PCR). M(C) rats had significantly lower transforming growth factor (TGF-(1)) mRNA levels than E(C) rats (258 +/- 083 versus 353 +/- 057, P < 001) and in comparison with M(S) and M(A) rats (466 +/- 249 and 461 +/- 211, respectively; P < 005). VEGF and KGF-7 mRNA levels were lower in M(A) rats than in E(A) rats (074 +/- 016 versus 125 +/- 066; and 107 +/- 045 versus 179 +/- 089, respectively; P 004), but did not differ from levels in E(C) and M(C) animals. in experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF-(1) genes, which was minimised by early enteral feeding with standard or supplemented diets.
Keywords Antioxidants
Arginine
Enteral nutrition
Growth factors
TGF-(1)
Wound healing
Language English
Sponsor Fundacao de Apoio a Pesquisa do Estado de São Paulo
Grant number Fundacao de Apoio a Pesquisa do Estado de São Paulo: Fapesp 05/54185-5
Date 2014-10-01
Published in International Wound Journal. Hoboken: Wiley-Blackwell, v. 11, n. 5, p. 533-539, 2014.
ISSN 1742-4801 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 533-539
Origin http://dx.doi.org/10.1111/j.1742-481X.2012.01120.x
Access rights Closed access
Type Article
Web of Science ID WOS:000342664900012
URI http://repositorio.unifesp.br/handle/11600/38310

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