Green tea extract improves high fat diet-induced hypothalamic inflammation, without affecting the serotoninergic system

Green tea extract improves high fat diet-induced hypothalamic inflammation, without affecting the serotoninergic system

Author Okuda, Marcos H. Autor UNIFESP Google Scholar
Zemdegs, Juliane C. S. Autor UNIFESP Google Scholar
Santana, Aline A. de Autor UNIFESP Google Scholar
Santamarina, Aline B. Autor UNIFESP Google Scholar
Moreno, Mayara F. Autor UNIFESP Google Scholar
Hachul, Ana C. L. Autor UNIFESP Google Scholar
Santos, Bruno dos Autor UNIFESP Google Scholar
Oller do Nascimento, Claudia M. Autor UNIFESP Google Scholar
Ribeiro, Eliane B. Autor UNIFESP Google Scholar
Oyama, Lila M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract To investigate possible mechanisms of green tea's anti-obesity and anti-diabetic effects in the hypothalamus, the central regulator of metabolism, of mice fed with high-fat diet (HFD), we analyzed proteins of the toll-like receptor 4 (TLR4) pathway and serotoninergic proteins involved in energy homeostasis. Thirty-day-old male Swiss mice were fed with HFD rich in saturated fat and green tea extract (GTE) for 8 weeks. After that, body weight and mass of fat depots were evaluated. Oral glucose tolerance test was performed 3 days prior to euthanasia; serum glucose, insulin and adiponectin were measured in fasted mice. Hypothalamic TLR4 pathway proteins, serotonin receptors 1B and 2C and serotonin transporter were analyzed by Western blotting or enzyme-linked immunosorbent assay. A second set of animals was used to measure food intake in response to fluoxetine, a selective serotonin reuptake inhibitor. Mice fed with HFD had increased body weight and mass of fat depots, impaired oral glucose tolerance, elevated glucose and insulin and decreased adiponectin serum levels. TLR4, I kappa B-alpha, nuclear factor kappa B p50 and interleukin 6 were increased by HFD. Concomitant GTE treatment ameliorated these parameters. the serotoninergic system remained functional after HFD treatment despite a few alterations in protein content of serotonin receptors I B and 2C and serotonin transporter. in summary, the GTE attenuated the deleterious effects of the HFD investigated in this study, partially due to reduced hypothalamic inflammation. (C) 2014 Elsevier Inc. All rights reserved.
Keywords Green tea extract
High-fat diet
Hypothalamus
Neuroinflammation
Serotonin
Language English
Sponsor DSM Nutritional Products Ltd., Basel, Switzerland
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2009/14373-8
FAPESP: 2011/16199-5
FAPESP: 2012/03713-5
Date 2014-10-01
Published in Journal of Nutritional Biochemistry. New York: Elsevier B.V., v. 25, n. 10, p. 1084-1089, 2014.
ISSN 0955-2863 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1084-1089
Origin http://dx.doi.org/10.1016/j.jnutbio.2014.05.012
Access rights Closed access
Type Article
Web of Science ID WOS:000341799200012
URI http://repositorio.unifesp.br/handle/11600/38290

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