Modulation of inflammation response to murine cutaneous Leishmaniasis by homeopathic medicines: Antimonium crudum 30cH

Show simple item record Santana, Fabiana Rodrigues de Coelho, Cideli de Paula Cardoso, Thayna Neves Pérez, Elizabeth Cristina [UNIFESP] Benites, Nilson Roberti Laurenti, Marcia Dalastra Bonamin, Leoni Villano 2016-01-24T14:37:57Z 2016-01-24T14:37:57Z 2014-10-01
dc.identifier.citation Homeopathy. Oxford: Elsevier B.V., v. 103, n. 4, p. 264-274, 2014.
dc.identifier.issn 1475-4916
dc.description.abstract Background: Leishmaniasis is a zoonotic disease caused by protozoan parasites of the mononuclear phagocytic system. the modulation activity of these cells can interfere in the host/parasite relationship and influences the prognosis.Methods: We evaluated the effects of the homeopathic preparation Antimonium crudum 30cH on experimental infection induced by Leishmania (L.) amazonensis. Male Balb/c mice were inoculated with 2 x 10(6) Leishmania (L.) amazonensis promastigotes into the footpad and, after 48 h (acute phase) or 60 days (chronic phase), cell population of lymphocytes and phagocytes present in the peritoneal washing fluid and spleen were analyzed by flow cytometry and histopathology, with histometry of the subcutaneous primary lesion, local lymph node and spleen. Immunohistochemistry was performed to quantify CD3 (T lymphocyte), CD45RA (B lymphocyte) and CD11b (phagocytes) positive cells.Results: in treated mice, during the acute phase, there was significant increase of the macroscopic lesion, associated to inflammatory edema, as well increase in the number of free amastigotes and B lymphocytes inside the lesion. Increase of B lymphocytes (predominantly B-2 cells) was also seen in the local lymph node, spleen and peritoneum. in the chronic phase, the inflammatory process in the infection focus was reduced, with reduced phagocyte migration and peritoneal increase of B-1a cells (precursors of B-2 immunoglobulin producers cells) and T CD8+ cells.Conclusion: the treatment of mice with Antimonium crudum 30cH induced a predominantly B cell pattern of immune response in Leishmania (L.) amazonensis experimental infection, alongside the increase of free amastigote forms number in the infection site. the clinical significance of this study is discussed, further studies are suggested. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship UNIP
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 264-274
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Homeopathy
dc.rights Acesso restrito
dc.subject Leishmania (L.) amazonensis en
dc.subject Antimoniurn crudum en
dc.subject Homeopathy en
dc.subject Murine leishnnaniasis en
dc.title Modulation of inflammation response to murine cutaneous Leishmaniasis by homeopathic medicines: Antimonium crudum 30cH en
dc.type Artigo
dc.contributor.institution Univ Paulista
dc.contributor.institution Univ Santo Amara
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Univ Paulista, Res Ctr, Grad Program Environm & Expt Pathol, BR-04026002 São Paulo, Brazil
dc.description.affiliation Univ Santo Amara, Lab Vet Pathol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Immunol Lab, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Vet & Zootechny Fac, BR-05508 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Med, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Immunol Lab, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2010/50842-0
dc.identifier.doi 10.1016/j.homp.2014.08.006
dc.description.source Web of Science
dc.identifier.wos WOS:000345058100008


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