Modulation of inflammation response to murine cutaneous Leishmaniasis by homeopathic medicines: Antimonium crudum 30cH

Modulation of inflammation response to murine cutaneous Leishmaniasis by homeopathic medicines: Antimonium crudum 30cH

Author Santana, Fabiana Rodrigues de Google Scholar
Coelho, Cideli de Paula Google Scholar
Cardoso, Thayna Neves Google Scholar
Pérez, Elizabeth Cristina Autor UNIFESP Google Scholar
Benites, Nilson Roberti Google Scholar
Laurenti, Marcia Dalastra Google Scholar
Bonamin, Leoni Villano Google Scholar
Institution Univ Paulista
Univ Santo Amara
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Background: Leishmaniasis is a zoonotic disease caused by protozoan parasites of the mononuclear phagocytic system. the modulation activity of these cells can interfere in the host/parasite relationship and influences the prognosis.Methods: We evaluated the effects of the homeopathic preparation Antimonium crudum 30cH on experimental infection induced by Leishmania (L.) amazonensis. Male Balb/c mice were inoculated with 2 x 10(6) Leishmania (L.) amazonensis promastigotes into the footpad and, after 48 h (acute phase) or 60 days (chronic phase), cell population of lymphocytes and phagocytes present in the peritoneal washing fluid and spleen were analyzed by flow cytometry and histopathology, with histometry of the subcutaneous primary lesion, local lymph node and spleen. Immunohistochemistry was performed to quantify CD3 (T lymphocyte), CD45RA (B lymphocyte) and CD11b (phagocytes) positive cells.Results: in treated mice, during the acute phase, there was significant increase of the macroscopic lesion, associated to inflammatory edema, as well increase in the number of free amastigotes and B lymphocytes inside the lesion. Increase of B lymphocytes (predominantly B-2 cells) was also seen in the local lymph node, spleen and peritoneum. in the chronic phase, the inflammatory process in the infection focus was reduced, with reduced phagocyte migration and peritoneal increase of B-1a cells (precursors of B-2 immunoglobulin producers cells) and T CD8+ cells.Conclusion: the treatment of mice with Antimonium crudum 30cH induced a predominantly B cell pattern of immune response in Leishmania (L.) amazonensis experimental infection, alongside the increase of free amastigote forms number in the infection site. the clinical significance of this study is discussed, further studies are suggested.
Keywords Leishmania (L.) amazonensis
Antimoniurn crudum
Murine leishnnaniasis
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2010/50842-0
Date 2014-10-01
Published in Homeopathy. Oxford: Elsevier B.V., v. 103, n. 4, p. 264-274, 2014.
ISSN 1475-4916 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 264-274
Access rights Closed access
Type Article
Web of Science ID WOS:000345058100008

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