Oral microbial colonization in children with sickle cell anaemia under long-term prophylaxis with penicillin

Oral microbial colonization in children with sickle cell anaemia under long-term prophylaxis with penicillin

Author Matos, Bruno Mello de Google Scholar
Abreu Ribeiro, Zulene Eveline Google Scholar
Balducci, Ivan Google Scholar
Figueiredo, Maria Stella Autor UNIFESP Google Scholar
Back-Brito, Graziella Nuernberg Google Scholar
Mota, Adolfo Jose da Google Scholar
Pellegrini Braga, Josefina Aparecida Google Scholar
Koga-Ito, Cristiane Yumi Google Scholar
Institution Univ Estadual Paulista
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Amazonas
Abstract Background and objective: Sickle cell anaemia (SCA) is the most frequent haematological hereditary disease. Children with SCA are submitted to long-term prophylactic therapy with penicillin, but little is known about its impact on oral microflora. the aim of this study was to evaluate the oral microbial colonization of paediatric patients with SCA.Design: Forty children (4-11 yrs old) with SCA (genotype SS) under long-term prophylactic treatment with penicillin were included in the study. Age/gender-matched control group of healthy children was also included. Scores of dmft/DMFT (number of decayed (D), missing (M), or filled (F) teeth; dmft, for primary dentition; DMFT, for permanent dentition) were obtained and stimulated saliva was sampled. Salivary flow rate and buffering capacity were evaluated. Counts of microorganisms (mutans streptococci, lactobacilli and yeasts) were determined by plating method. Yeasts were identified by API 20C AUX and PCR.Results: Mean dmft/DMFT values were similar in the studied groups (SCA 2.13/1.60 and control 2.38/1.3). Although no significant differences between cariogenic microorganism counts were observed, significantly higher yeasts oral levels were observed in SCA group. Controls showed lower salivary buffering capacity. Candida albicans was the most frequently isolated species in both groups. Candida famata, Candida parapsilosis and Candida tropical is were also isolated from controls. Candida dubliniensis, Candida rugosa and Candida sphaerica were found only in SCA group.Conclusions: Based on the results, it could be concluded that paediatric patients with SCA showed significantly higher oral level of yeasts. Uncommon fungal species were found in SCA group. Similar caries prevalence and counts of lactobacilli and streptococci in relation to controls were observed. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Sickle cell anaemia
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 07/58999-2
Date 2014-10-01
Published in Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V., v. 59, n. 10, p. 1042-1047, 2014.
ISSN 0003-9969 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1042-1047
Origin http://dx.doi.org/10.1016/j.archoralbio.2014.05.014
Access rights Closed access
Type Article
Web of Science ID WOS:000341481700005
URI http://repositorio.unifesp.br/handle/11600/38279

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