MMP-9 Expression Increases According to the Grade of Squamous Intraepithelial Lesion in Cervical Smears

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dc.contributor.author Matheus, Erika R.
dc.contributor.author Zonta, Marco A.
dc.contributor.author Discacciati, Michelle G.
dc.contributor.author Paruci, Priscila
dc.contributor.author Velame, Fernanda
dc.contributor.author Cardeal, Laura B. S.
dc.contributor.author Barros, Silvia B. M.
dc.contributor.author Pignatari, Antonio C. [UNIFESP]
dc.contributor.author Maria-Engler, Silvya S.
dc.date.accessioned 2016-01-24T14:37:54Z
dc.date.available 2016-01-24T14:37:54Z
dc.date.issued 2014-10-01
dc.identifier http://dx.doi.org/10.1002/dc.23124
dc.identifier.citation Diagnostic Cytopathology. Hoboken: Wiley-Blackwell, v. 42, n. 10, p. 827-833, 2014.
dc.identifier.issn 8755-1039
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38260
dc.description.abstract Studies about cervical carcinogenesis have demonstrated the increased expression of matrix-metalloproteinase (MMP) according to the grade of cervical intraepithelial lesions. Considering the importance of innovative techniques to introduce noninvasive and rapid diagnoses for patients, this study aimed to perform MMP-9 immunocytochemistry in cervical smears according to the cytopathological diagnoses, in order to monitor MMP activity in cervical smears. This cross-sectional study investigated the expression of MMP-9 in normal cervical smears, inflammatory cervical smears, squamous intraepithelial lesions, and cervical carcinoma. Cervical smears from 630 women were collected for cytopathological diagnoses and immunocytochemistry. Women with squamous intraepithelial lesions showed an increase in MMP-9 expression, with moderate to intense staining occurring with increasing cervical lesion grade. the prevalence of moderate to intense MMP-9 staining was 9% in normal cervical smears, 12% in cervical inflammation, 24% in low-grade squamous intraepithelial lesion (LSIL), 92% in high-grade squamous intraepithelial lesions (HSIL) and 100% in cervical carcinoma cases. in the specific case of LSIL, we found that association with MMP-9 is more evident when there is the simultaneous presence of an infectious agent. Thus, the expression of MMP-9 in cervical smears increases according to the grade of cervical lesion and LSIL in the presence of infectious agents showed higher MMP-9 expression than women with LSIL without infectious agents. Diagn. Cytopathol. 2014;42:827-833. (c) 2014 Wiley Periodicals, Inc. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Pro-Reitoria de Pos-Graduacao da Universidade de São Paulo (PRPG-USP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship INCT_if
dc.format.extent 827-833
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Diagnostic Cytopathology
dc.rights Acesso restrito
dc.subject matrix metalloproteinase 9 en
dc.subject cervical smear en
dc.subject cervical cancer en
dc.subject human papillomavirus en
dc.subject cervical intraepithelial neoplasia en
dc.subject genital tract infections en
dc.subject inflammation en
dc.subject uterine cervical cancer en
dc.subject MMP-9 en
dc.subject pap smear en
dc.title MMP-9 Expression Increases According to the Grade of Squamous Intraepithelial Lesion in Cervical Smears en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution In Cito Lab Diagnost Cytol
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Sch Pharmaceut Sci, Dept Clin Chem & Toxicol, Lab Clin Pathol & Cytol, BR-05508000 São Paulo, Brazil
dc.description.affiliation In Cito Lab Diagnost Cytol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Med, Special Lab Clin Microbiol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Med, Special Lab Clin Microbiol, BR-05508000 São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2008/58817-4
dc.description.sponsorshipID FAPESP: 2012/09746-2
dc.description.sponsorshipID INCT_if: 573663/2008-4
dc.identifier.doi 10.1002/dc.23124
dc.description.source Web of Science
dc.identifier.wos WOS:000342690200001



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