Anti-tumor activities of peptides corresponding to conserved complementary determining regions from different immunoglobulins

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dc.contributor.author Figueiredo, Carlos R. [UNIFESP]
dc.contributor.author Matsuo, Alisson L. [UNIFESP]
dc.contributor.author Massaoka, Mariana H. [UNIFESP]
dc.contributor.author Polonelli, Luciano
dc.contributor.author Travassos, Luiz R. [UNIFESP]
dc.date.accessioned 2016-01-24T14:37:49Z
dc.date.available 2016-01-24T14:37:49Z
dc.date.issued 2014-09-01
dc.identifier http://dx.doi.org/10.1016/j.peptides.2014.06.007
dc.identifier.citation Peptides. New York: Elsevier B.V., v. 59, p. 14-19, 2014.
dc.identifier.issn 0196-9781
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38174
dc.description.abstract Short synthetic peptides corresponding to sequences of complementarity-determining regions (CDRs) from different immunoglobulin families have been shown to induce antimicrobial, antiviral and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). Presently, we studied the in vitro and in vivo antitumor activity of synthetic peptides derived from conserved CDR sequences of different immunoglobulins against human tumor cell lines and murine B16F10-Nex2 melanoma aiming at the discovery of candidate molecules for cancer therapy. Four light-and heavy-chain CDR peptide sequences from different antibodies (C36-L1, HA9-H2, 1-H2 and Mg16-H2) showed cytotoxic activity against murine melanoma and a panel of human tumor cell lineages in vitro. Importantly, theyalso exerted anti-metastatic activity using a syngeneic melanoma model in mice. Other peptides (D07-H3, MN20v1, MS2-H3) were also protective against metastatic melanoma, without showing significant cytotoxicity against tumor cells in vitro. in this case, we suggest that these peptides may act as immune adjuvants in vivo. As observed, peptides induced nitric oxide production in bone-marrow macrophages showing that innate immune cells can also be modulated by these CDR peptides. the present screening supports the search in immunoglobulins of rather frequent CDR sequences that are endowed with specific antitumor properties and may be candidates to be developed as anti-cancer drugs. (C) 2014 Elsevier Inc. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 14-19
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Peptides
dc.rights Acesso restrito
dc.subject Peptide en
dc.subject CDR en
dc.subject Cytotoxicity en
dc.subject Antitumor activity en
dc.subject Melanoma en
dc.title Anti-tumor activities of peptides corresponding to conserved complementary determining regions from different immunoglobulins en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Parma
dc.contributor.institution Recepta Biopharma
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, São Paulo, SP, Brazil
dc.description.affiliation Univ Parma, Dept Biomed Biotechnol & Translat Sci, Microbiol & Virol Unit, I-43121 Parma, Italy
dc.description.affiliation Recepta Biopharma, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, São Paulo, SP, Brazil
dc.description.sponsorshipID FAPESP: 2010/51423-0
dc.identifier.doi 10.1016/j.peptides.2014.06.007
dc.description.source Web of Science
dc.identifier.wos WOS:000341427300003



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