MALDI-TOF Fingerprinting of Seminal Plasma Lipids in the Study of Human Male Infertility

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dc.contributor.author Camargo, Mariana [UNIFESP]
dc.contributor.author Intasqui, Paula [UNIFESP]
dc.contributor.author Lima, Camila Bruna de [UNIFESP]
dc.contributor.author Montani, Daniela Antunes [UNIFESP]
dc.contributor.author Nichi, Marcilio
dc.contributor.author Pilau, Eduardo Jorge
dc.contributor.author Gozzo, Fabio Cesar
dc.contributor.author Lo Turco, Edson Guimaraes [UNIFESP]
dc.contributor.author Bertolla, Ricardo Pimenta [UNIFESP]
dc.date.accessioned 2016-01-24T14:37:47Z
dc.date.available 2016-01-24T14:37:47Z
dc.date.issued 2014-09-01
dc.identifier http://dx.doi.org/10.1007/s11745-014-3922-7
dc.identifier.citation Lipids. Heidelberg: Springer Heidelberg, v. 49, n. 9, p. 943-956, 2014.
dc.identifier.issn 0024-4201
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38143
dc.description.abstract This study proposed lipid fingerprinting of human seminal plasma by mass spectrometry as an analytical method to differentiate biological conditions. for this purpose, we chose infertile men as a model to study specific conditions, namely: high and low seminal plasma lipid peroxidation levels (sub-study 1.1), high and low sperm nuclear DNA fragmentation (sub-study 1.2), and intervention status: before and after subinguinal microsurgical varicocelectomy (study 2). Study 1 included 133 patients, of which 113 were utilized for sub-study 1.1 and 89 for sub-study 1.2. Study 2 included 17 adult men submitted to subinguinal varicocelectomy, before and 90 days after varicocelectomy. Lipids were extracted from seminal plasma and submitted to Matrix-Assisted Laser Desorption Ionization Quadrupole-Time-of-Flight Mass Spectrometry in the positive ionization mode. Spectra were processed using Waters(A (R)) MassLynx, and MetaboAnalyst online software was used for statistical analyses. for sub-studies 1.1 and 1.2, and study 2, univariate analysis revealed 8, 87 and 34 significant ions, respectively. Multivariate analysis was performed through PCA and PLS-DA. PCA generated 56, 32 and 34 components respectively for each study and these were submitted to logistic regression. A ROC curve was plotted and the area under the curve was equal to 97.4, 92.5 and 96.5 %. PLS-DA generated a list of 19, 24 and 23 VIP ions for sub-studies 1.1 and 1.2, and study 2, respectively. Therefore, this study established the lipid profile and comparison of patterns altered in response to specific biological conditions. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 943-956
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof Lipids
dc.rights Acesso restrito
dc.subject DNA fragmentation en
dc.subject Lipid fingerprinting en
dc.subject Lipid peroxidation en
dc.subject Mass spectrometry en
dc.subject Semen en
dc.subject Spermatozoa en
dc.subject Varicocelectomy en
dc.title MALDI-TOF Fingerprinting of Seminal Plasma Lipids in the Study of Human Male Infertility en
dc.type Artigo
dc.rights.license http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Estadual de Campinas (UNICAMP)
dc.contributor.institution Natl Inst Sci & Technol Bioanalyt
dc.contributor.institution Universidade Estadual de Maringá (UEM)
dc.description.affiliation Universidade Federal de São Paulo, Human Reprod Sect, Dept Surg, Div Urol, BR-04039060 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Sch Vet Med, Dept Anim Reprod, São Paulo, Brazil
dc.description.affiliation Univ Estadual Campinas, Inst Chem, São Paulo, Brazil
dc.description.affiliation Natl Inst Sci & Technol Bioanalyt, São Paulo, Brazil
dc.description.affiliation Univ Estadual Maringa, Ctr Exact Sci, Dept Chem, Maringa, PR, Brazil
dc.description.affiliation Hosp São Paulo, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Human Reprod Sect, Dept Surg, Div Urol, BR-04039060 São Paulo, Brazil
dc.description.affiliationUnifesp Hosp São Paulo, São Paulo, Brazil
dc.description.sponsorshipID CNPq: 472941/2012-7
dc.identifier.doi 10.1007/s11745-014-3922-7
dc.description.source Web of Science
dc.identifier.wos WOS:000341085800008



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