MALDI-TOF Fingerprinting of Seminal Plasma Lipids in the Study of Human Male Infertility

MALDI-TOF Fingerprinting of Seminal Plasma Lipids in the Study of Human Male Infertility

Author Camargo, Mariana Autor UNIFESP Google Scholar
Intasqui, Paula Autor UNIFESP Google Scholar
Lima, Camila Bruna de Autor UNIFESP Google Scholar
Montani, Daniela Antunes Autor UNIFESP Google Scholar
Nichi, Marcilio Google Scholar
Pilau, Eduardo Jorge Google Scholar
Gozzo, Fabio Cesar Google Scholar
Lo Turco, Edson Guimaraes Autor UNIFESP Google Scholar
Bertolla, Ricardo Pimenta Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Universidade Estadual de Campinas (UNICAMP)
Natl Inst Sci & Technol Bioanalyt
Universidade Estadual de Maringá (UEM)
Abstract This study proposed lipid fingerprinting of human seminal plasma by mass spectrometry as an analytical method to differentiate biological conditions. for this purpose, we chose infertile men as a model to study specific conditions, namely: high and low seminal plasma lipid peroxidation levels (sub-study 1.1), high and low sperm nuclear DNA fragmentation (sub-study 1.2), and intervention status: before and after subinguinal microsurgical varicocelectomy (study 2). Study 1 included 133 patients, of which 113 were utilized for sub-study 1.1 and 89 for sub-study 1.2. Study 2 included 17 adult men submitted to subinguinal varicocelectomy, before and 90 days after varicocelectomy. Lipids were extracted from seminal plasma and submitted to Matrix-Assisted Laser Desorption Ionization Quadrupole-Time-of-Flight Mass Spectrometry in the positive ionization mode. Spectra were processed using Waters(A (R)) MassLynx, and MetaboAnalyst online software was used for statistical analyses. for sub-studies 1.1 and 1.2, and study 2, univariate analysis revealed 8, 87 and 34 significant ions, respectively. Multivariate analysis was performed through PCA and PLS-DA. PCA generated 56, 32 and 34 components respectively for each study and these were submitted to logistic regression. A ROC curve was plotted and the area under the curve was equal to 97.4, 92.5 and 96.5 %. PLS-DA generated a list of 19, 24 and 23 VIP ions for sub-studies 1.1 and 1.2, and study 2, respectively. Therefore, this study established the lipid profile and comparison of patterns altered in response to specific biological conditions.
Keywords DNA fragmentation
Lipid fingerprinting
Lipid peroxidation
Mass spectrometry
Semen
Spermatozoa
Varicocelectomy
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number CNPq: 472941/2012-7
Date 2014-09-01
Published in Lipids. Heidelberg: Springer Heidelberg, v. 49, n. 9, p. 943-956, 2014.
ISSN 0024-4201 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 943-956
Origin http://dx.doi.org/10.1007/s11745-014-3922-7
Access rights Closed access
Type Article
Web of Science ID WOS:000341085800008
URI http://repositorio.unifesp.br/handle/11600/38143

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