Low-level laser therapy prevents degenerative morphological changes in an experimental model of anterior cruciate ligament transection in rats

Low-level laser therapy prevents degenerative morphological changes in an experimental model of anterior cruciate ligament transection in rats

Author Bublitz, Caroline Autor UNIFESP Google Scholar
Medalha, Carla Autor UNIFESP Google Scholar
Oliveira, Poliani Autor UNIFESP Google Scholar
Assis, Livia Autor UNIFESP Google Scholar
Milares, Luiz Paulo Autor UNIFESP Google Scholar
Fernandes, Kelly Rossetti Autor UNIFESP Google Scholar
Tim, Carla Roberta Autor UNIFESP Google Scholar
Vasilceac, Fernando Augusto Autor UNIFESP Google Scholar
Mattiello, Stela Marcia Autor UNIFESP Google Scholar
Muniz Renno, Ana Claudia Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of this study was to analyze the effects of low-level laser therapy (LLLT) on the prevention of cartilage damage after the anterior cruciate ligament transection (ACLT) in knees of rats. Thirty male rats (Wistar) were distributed into three groups (n = 10 each): injured control group (CG); injured laser-treated group at 10 J/cm(2) (L10), and injured laser-treated group at 50 J/cm(2) (L50). Laser treatment started immediately after the surgery and it was performed for 15 sessions. An 808 nm laser, at 10 and 50 J/cm(2), was used. To evaluate the effects of LLLT, the qualitative and semi-quantitative histological, morphometric, and immunohistochemistry analysis were performed. Initial signs of tissue degradation were observed in CG. Interestingly, laser-treated animals presented a better tissue organization, especially at the fluence of 10 J/cm(2). Furthermore, laser phototherapy was able of modulating some of the aspects related to the degenerative process, such as the prevention of proteoglycans loss and the increase in cartilage area. However, LLLT was not able of modulating chondrocytes proliferation and the immunoexpression of markers related to inflammatory process (IL-1 and MMP-13). This study showed that 808 nm laser, at both fluences, prevented features related to the articular degenerative process in the knees of rats after ACLT.
Keywords LLLT
Articular cartilage
Proteoglycan
Metalloproteinase 13
Interleukin-1
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2014-09-01
Published in Lasers in Medical Science. London: Springer London Ltd, v. 29, n. 5, p. 1669-1678, 2014.
ISSN 0268-8921 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1669-1678
Origin http://dx.doi.org/10.1007/s10103-014-1546-z
Access rights Closed access
Type Article
Web of Science ID WOS:000341503500020
URI http://repositorio.unifesp.br/handle/11600/38140

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