Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions

Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions

Autor Mattos, Viviane Fagundes Google Scholar
Carvalho, Leonardo Sousa Google Scholar
Cella, Doralice Maria Google Scholar
Schneider, Marielle Cristina Autor UNIFESP Google Scholar
Instituição Univ Estadual Paulista
Univ Fed Piaui
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Resumo Buthid scorpions exhibit a high variability in diploid number within genera and even within species. Cytogenetically, Buthidae differs from other families of Scorpiones based on its low diploid numbers, holocentric chromosomes, and complex chromosomal chains, which form during meiosis. in this study, we analyzed the distribution of the 45S ribosomal DNA (rDNA) genes in the mitotic and meiotic chromosomes of seven buthid species belonging to the genera Rhopalurus and Tityus with the ultimate goal of elucidating the chromosome organization in these scorpions. the chromosome number ranged from 2n = 6 to 2n = 28. Despite the high variance in diploid number, all species examined carried their 45S rDNA sites in the terminal region of exactly two chromosomes. Analyses of meiotic cells revealed 45S rDNA clusters in the chromosomal chains of Rhopalurus agamemnon, Tityus bahiensis, Tityus confluens, and Tityus martinpaechi, or in bivalent-like configuration in Rhopalurus rochai, Tityus bahiensis, Tityus confluens, Tityus fasciolatus, and Tityus paraguayensis. in the species examined, the 45S rDNA sites colocalized with constitutive heterochromatin regions. in light of the high chromosome variability and maintenance of number and terminal position of 45S rDNA sites in buthids, the heterochromatin may act to conserve the integrity of the ribosomal genes.
Palavra-chave constitutive heterochromatin
chromosome chain
diploid number
fluorescent in situ hybridization
holocentric chromosomes
rDNA
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Programa de Pesquisas em Biodiversidade do Semiarido - PPBio Semiarido
Número do financiamento FAPESP: 2010/14226-2
FAPESP: 2011/21643-1
CNPq: 478630/2010-7
Programa de Pesquisas em Biodiversidade do Semiarido - PPBio Semiarido: CNPq 558317/2009-0
Programa de Pesquisas em Biodiversidade do Semiarido - PPBio Semiarido: 457471/2012-3
Data de publicação 2014-09-01
Publicado em Zoological Science. Tokyo: Zoological Soc Japan, v. 31, n. 9, p. 603-607, 2014.
ISSN 0289-0003 (Sherpa/Romeo, fator de impacto)
Publicador Zoological Soc Japan
Extensão 603-607
Fonte http://dx.doi.org/10.2108/zs140005
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000341573400008
Endereço permanente http://repositorio.unifesp.br/handle/11600/38133

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