Effects of different doses of soy isoflavones on bone tissue of ovariectomized rats

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dc.contributor.author Santos, M. A. [UNIFESP]
dc.contributor.author Florencio-Silva, R. [UNIFESP]
dc.contributor.author Medeiros, V. P. [UNIFESP]
dc.contributor.author Nader, H. B. [UNIFESP]
dc.contributor.author Nonaka, K. O.
dc.contributor.author Sasso, G. R. S. [UNIFESP]
dc.contributor.author Simoes, M. J. [UNIFESP]
dc.contributor.author Reginato, R. D. [UNIFESP]
dc.date.accessioned 2016-01-24T14:37:42Z
dc.date.available 2016-01-24T14:37:42Z
dc.date.issued 2014-08-01
dc.identifier http://dx.doi.org/10.3109/13697137.2013.830606
dc.identifier.citation Climacteric. London: Informa Healthcare, v. 17, n. 4, p. 393-401, 2014.
dc.identifier.issn 1369-7137
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38074
dc.description.abstract Aim Studies report that hormone replacement prevents osteoporosis, but there are doubts whether isoflavones are really efficient in this process. the aim of this study was to evaluate the effects of different doses of soy isoflavones on bone tissue of ovariectomized rats.Methods Forty female rats at the age of 6 months were ovariectomized and, after 3 months, the animals were divided into four groups: GI - Control (treated with drug vehicle); GII - treated with isoflavones (80 mg/kg per day); GIII - treated with isoflavones (200 mg/kg per day) and GIV - treated with isoflavones (350 mg/kg per day). Soy isoflavones were administered by gavage for 90 consecutive days. After treatment, the rats were euthanized and their distal femurs were removed for histological routine, histochemistry and biochemical study. Histological sections were stained with hematoxylin-eosin or subjected to picrosirius red and alcian blue methods. Shafts of femurs were submitted to biochemical assay and tibias were subjected to biophysical and biomechanical tests.Results in distal femurs, the trabecular bone volume was higher in the groups treated with isoflavones, being higher in GIV, while the cortical bone width and the presence of mature type I collagen fibers were higher in GII. At the trabecular bone region, the percentage of total glycosaminoglycans (GAGs) was higher in GII and the percentage of only sulfated GAGs was higher in Gill, while the higher content of chondroitin sulfate in shafts of femurs was seen in GIV. Biophysical and biomechanical tests in tibias did not differ among the groups.Conclusion Our data indicate that soy isoflavones improve bone quality in femurs of rats by increasing histomorphometric parameters, the content of GAGs and mature type I collagen fibers. These positive effects are dose-dependent and it was different in cortical and trabecular bone. en
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 393-401
dc.language.iso eng
dc.publisher Informa Healthcare
dc.relation.ispartof Climacteric
dc.rights Acesso restrito
dc.subject ISOFLAVONES en
dc.subject BONE en
dc.subject COLLAGEN en
dc.subject RATS en
dc.title Effects of different doses of soy isoflavones on bone tissue of ovariectomized rats en
dc.type Artigo
dc.rights.license http://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal de São Carlos (UFSCar)
dc.description.affiliation Universidade Federal de São Paulo, BR-04021001 São Paulo, Brazil
dc.description.affiliation Univ Fed Sao Carlos, BR-13560 Sao Carlos, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, BR-04021001 São Paulo, Brazil
dc.identifier.doi 10.3109/13697137.2013.830606
dc.description.source Web of Science
dc.identifier.wos WOS:000339698900012


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