Effects of different doses of soy isoflavones on bone tissue of ovariectomized rats

Effects of different doses of soy isoflavones on bone tissue of ovariectomized rats

Author Santos, M. A. Autor UNIFESP Google Scholar
Florencio-Silva, R. Autor UNIFESP Google Scholar
Medeiros, V. P. Autor UNIFESP Google Scholar
Nader, H. B. Autor UNIFESP Google Scholar
Nonaka, K. O. Google Scholar
Sasso, G. R. S. Autor UNIFESP Google Scholar
Simoes, M. J. Autor UNIFESP Google Scholar
Reginato, R. D. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de São Carlos (UFSCar)
Abstract Aim Studies report that hormone replacement prevents osteoporosis, but there are doubts whether isoflavones are really efficient in this process. the aim of this study was to evaluate the effects of different doses of soy isoflavones on bone tissue of ovariectomized rats.Methods Forty female rats at the age of 6 months were ovariectomized and, after 3 months, the animals were divided into four groups: GI - Control (treated with drug vehicle); GII - treated with isoflavones (80 mg/kg per day); GIII - treated with isoflavones (200 mg/kg per day) and GIV - treated with isoflavones (350 mg/kg per day). Soy isoflavones were administered by gavage for 90 consecutive days. After treatment, the rats were euthanized and their distal femurs were removed for histological routine, histochemistry and biochemical study. Histological sections were stained with hematoxylin-eosin or subjected to picrosirius red and alcian blue methods. Shafts of femurs were submitted to biochemical assay and tibias were subjected to biophysical and biomechanical tests.Results in distal femurs, the trabecular bone volume was higher in the groups treated with isoflavones, being higher in GIV, while the cortical bone width and the presence of mature type I collagen fibers were higher in GII. At the trabecular bone region, the percentage of total glycosaminoglycans (GAGs) was higher in GII and the percentage of only sulfated GAGs was higher in Gill, while the higher content of chondroitin sulfate in shafts of femurs was seen in GIV. Biophysical and biomechanical tests in tibias did not differ among the groups.Conclusion Our data indicate that soy isoflavones improve bone quality in femurs of rats by increasing histomorphometric parameters, the content of GAGs and mature type I collagen fibers. These positive effects are dose-dependent and it was different in cortical and trabecular bone.
Keywords ISOFLAVONES
BONE
COLLAGEN
GLYCOSAMINOGLYCANS
RATS
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-08-01
Published in Climacteric. London: Informa Healthcare, v. 17, n. 4, p. 393-401, 2014.
ISSN 1369-7137 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 393-401
Origin http://dx.doi.org/10.3109/13697137.2013.830606
Access rights Closed access
Type Article
Web of Science ID WOS:000339698900012
URI http://repositorio.unifesp.br/handle/11600/38074

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