Effect of natural porcine surfactant in Staphylococcus aureus induced pro-inflammatory cytokines and reactive oxygen species generation in monocytes and neutrophils from human blood

Effect of natural porcine surfactant in Staphylococcus aureus induced pro-inflammatory cytokines and reactive oxygen species generation in monocytes and neutrophils from human blood

Author Guevara, Yuliannis Lugones Ladron de Google Scholar
Hidalgo, Odalys Blanco Google Scholar
Santos, Sidneia Sousa Autor UNIFESP Google Scholar
Brunialti, Milena Karina Coló Autor UNIFESP Google Scholar
Faure, Roberto Google Scholar
Salomão, Reinaldo Autor UNIFESP Google Scholar
Institution Ctr Nacl Sanidad Agr CENSA
Universidade Federal de São Paulo (UNIFESP)
Abstract Surfacen (R) is a clinical surfactant preparation of porcine origin. in the present study, we have evaluated the effect of Surfacen (R) in the modulation of oxidative burst in monocytes and neutrophils in human blood and proinflammatory cytokine production in peripheral blood mononuclear cells (PBMC). Reactive oxygen species (ROS) level was measured in monocytes and neutrophils by flow cytometry using 2,7-dichlorofluorescein diacetate (DCFH-DA) as substrate, while, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 levels were estimated in PBMC supernatant by enzyme-linked immunosorbent assays (ELISA). Our results show that Staphylococcus aureus-induced ROS level was slightly affected by Surfacen (R) added to whole blood monocytes and neutrophils. the time course experiments of pre-incubation with Surfacen (R) showed no significant increase of ROS level at 2 h; however, the ROS level decreased when pre incubated for 4 h and 6 h with Surfacen (R). Pre-incubation of PBMC cells with Surfacen (R) at 0.125 and 0.5 mg/mL showed a dose-dependent suppression of TNF-alpha levels measured after 4 h of S. aureus stimulation, an effect less impressive when cells were stimulated for 24 h. A similar behavior was observed in IL-6 release. in summary, the present study provides experimental evidence supporting an anti-inflammatory role of Surfacen (R) in human monocytes and neutrophils in vitro. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Surfacen (R)
Pulmonary surfactant
ROS
TNF-alpha
IL-6
Inflammation
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number CAPES: AUX-PE-MESCUBA: 103/10
Date 2014-08-01
Published in International Immunopharmacology. Amsterdam: Elsevier B.V., v. 21, n. 2, p. 369-374, 2014.
ISSN 1567-5769 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 369-374
Origin http://dx.doi.org/10.1016/j.intimp.2014.05.020
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000340319100016
URI http://repositorio.unifesp.br/handle/11600/38008

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