Effect of natural porcine surfactant in Staphylococcus aureus induced pro-inflammatory cytokines and reactive oxygen species generation in monocytes and neutrophils from human blood

Effect of natural porcine surfactant in Staphylococcus aureus induced pro-inflammatory cytokines and reactive oxygen species generation in monocytes and neutrophils from human blood

Autor Guevara, Yuliannis Lugones Ladron de Google Scholar
Hidalgo, Odalys Blanco Google Scholar
Santos, Sidneia Sousa Autor UNIFESP Google Scholar
Brunialti, Milena Karina Coló Autor UNIFESP Google Scholar
Faure, Roberto Google Scholar
Salomão, Reinaldo Autor UNIFESP Google Scholar
Instituição Ctr Nacl Sanidad Agr CENSA
Universidade Federal de São Paulo (UNIFESP)
Resumo Surfacen (R) is a clinical surfactant preparation of porcine origin. in the present study, we have evaluated the effect of Surfacen (R) in the modulation of oxidative burst in monocytes and neutrophils in human blood and proinflammatory cytokine production in peripheral blood mononuclear cells (PBMC). Reactive oxygen species (ROS) level was measured in monocytes and neutrophils by flow cytometry using 2,7-dichlorofluorescein diacetate (DCFH-DA) as substrate, while, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 levels were estimated in PBMC supernatant by enzyme-linked immunosorbent assays (ELISA). Our results show that Staphylococcus aureus-induced ROS level was slightly affected by Surfacen (R) added to whole blood monocytes and neutrophils. the time course experiments of pre-incubation with Surfacen (R) showed no significant increase of ROS level at 2 h; however, the ROS level decreased when pre incubated for 4 h and 6 h with Surfacen (R). Pre-incubation of PBMC cells with Surfacen (R) at 0.125 and 0.5 mg/mL showed a dose-dependent suppression of TNF-alpha levels measured after 4 h of S. aureus stimulation, an effect less impressive when cells were stimulated for 24 h. A similar behavior was observed in IL-6 release. in summary, the present study provides experimental evidence supporting an anti-inflammatory role of Surfacen (R) in human monocytes and neutrophils in vitro. (C) 2014 Elsevier B.V. All rights reserved.
Assunto Surfacen (R)
Pulmonary surfactant
ROS
TNF-alpha
IL-6
Inflammation
Idioma Inglês
Financiador Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Número do financiamento CAPES: AUX-PE-MESCUBA: 103/10
Data 2014-08-01
Publicado em International Immunopharmacology. Amsterdam: Elsevier B.V., v. 21, n. 2, p. 369-374, 2014.
ISSN 1567-5769 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 369-374
Fonte http://dx.doi.org/10.1016/j.intimp.2014.05.020
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000340319100016
URI http://repositorio.unifesp.br/handle/11600/38008

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