Amelioration of Cardiac Function and Activation of Anti-Inflammatory Vasoactive Peptides Expression in the Rat Myocardium by Low Level Laser Therapy

Amelioration of Cardiac Function and Activation of Anti-Inflammatory Vasoactive Peptides Expression in the Rat Myocardium by Low Level Laser Therapy

Author Manchini, Martha Trindade Google Scholar
Serra, Andrey Jorge Google Scholar
Feliciano, Regiane dos Santos Google Scholar
Santana, Eduardo Tadeu Google Scholar
Antonio, Ednei Luis Autor UNIFESP Google Scholar
Camillo de Carvalho, Paulo de Tarso Google Scholar
Montemor, Jairo Autor UNIFESP Google Scholar
Crajoinas, Renato Oliveira Google Scholar
Costa Girardi, Adriana Castello Google Scholar
Tucci, Paulo Jose Ferreira Autor UNIFESP Google Scholar
Silva, Jose Antonio Google Scholar
Institution Univ Nove Julho
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Low-level laser therapy (LLLT) has been used as an anti-inflammatory treatment in several disease conditions, even when inflammation is a secondary consequence, such as in myocardial infarction (MI). However, the mechanism by which LLLT is able to protect the remaining myocardium remains unclear. the present study tested the hypothesis that LLLT reduces inflammation after acute MI in female rats and ameliorates cardiac function. the potential participation of the Renin-Angiotensin System (RAS) and Kallikrein-Kinin System (KKS) vasoactive peptides was also evaluated. LLLT treatment effectively reduced MI size, attenuated the systolic dysfunction after MI, and decreased the myocardial mRNA expression of interleukin-1 beta and interleukin-6 in comparison to the non-irradiated rat tissue. in addition, LLLT treatment increased protein and mRNA levels of the Mas receptor, the mRNA expression of kinin B2 receptors and the circulating levels of plasma kallikrein compared to non-treated post-MI rats. On the other hand, the kinin B1 receptor mRNA expression decreased after LLLT. No significant changes were found in the expression of vascular endothelial growth factor (VEGF) in the myocardial remote area between laser-irradiated and non-irradiated post-MI rats. Capillaries density also remained similar between these two experimental groups. the mRNA expression of the inducible nitric oxide synthase (iNOS) was increased three days after MI, however, this effect was blunted by LLLT. Moreover, endothelial NOS mRNA content increased after LLLT. Plasma nitric oxide metabolites (NOx) concentration was increased three days after MI in non-treated rats and increased even further by LLLT treatment. Our data suggest that LLLT diminishes the acute inflammation in the myocardium, reduces infarct size and attenuates left ventricle dysfunction post-MI and increases vasoactive peptides expression and nitric oxide (NO) generation.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number CNPq: 477458/2009-2
CNPq: 309715/2011-3
CNPq: 479395/2012-8
: 2009/54225-8
Date 2014-07-03
Published in Plos One. San Francisco: Public Library Science, v. 9, n. 7, 10 p., 2014.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 10
Origin http://dx.doi.org/10.1371/journal.pone.0101270
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000341253400057
URI http://repositorio.unifesp.br/handle/11600/37984

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