Bauhinia forficata lectin (BfL) induces cell death and inhibits integrin-mediated adhesion on MCF7 human breast cancer cells

Bauhinia forficata lectin (BfL) induces cell death and inhibits integrin-mediated adhesion on MCF7 human breast cancer cells

Author Silva, Mariana C. C. Autor UNIFESP Google Scholar
Paula, Claudia A. A. de Autor UNIFESP Google Scholar
Ferreira, Joana G. Autor UNIFESP Google Scholar
Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Vaz, Angela M. S. F. Google Scholar
Sampaio, Misako U. Autor UNIFESP Google Scholar
Correia, Maria Tereza S. Google Scholar
Oliva, Maria Luiza V. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Pesquisas Jardim Bot Rio de Janeiro
Universidade Federal de Pernambuco (UFPE)
Abstract Background: Plant lectins have attracted great interest in cancer studies due to their antitumor activities. These proteins or glycoproteins specifically and reversibly bind to different types of carbohydrates or glycoproteins. Breast cancer, which presents altered glycosylation of cell surface glycoproteins, is one of the most frequent malignant diseases in women. in this work, we describe the effect of the lectin Bauhinia forficata lectin (BfL), which was purified from B. forficata Link subsp.forficata seeds, on the MCF7 human breast cancer cellular line, investigating the mechanisms involved in its antiproliferative activity.Methods: MCF7 cells were treated with BfL Viability and adhesion alterations were evaluated using flow cytometry and western blotting.Results: BfL. inhibited the viability of the MCF7 cell line but was ineffective on MDA-MB-231 and MCF 10A cells. It inhibits MCF7 adhesion on laminin, collagen I and fibronectin, decreases alpha(1), alpha(6) and beta(1) integrin subunit expression, and increases alpha(5) subunit expression. BfL triggers necrosis and secondary necrosis, with caspase-9 inhibition. It also causes deoxyribonucleic add (DNA) fragmentation, which leads to cell cycle arrest in the G2/M phase and a decrease in the expression of the regulatory proteins pRb and p21.Conclusion: BfL shows selective cytotoxic effect and adhesion inhibition on MCF7 breast cancer cells. General significance: Cell death induction and inhibition of cell adhesion may contribute to understanding the action of lectins in breast cancer. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Bauhinia foificata lectin
Breast cancer
Cell adhesion
Cell death
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 07/58929-4
FAPESP: 09/53766-5
FAPESP: 12/06366-4
CAPES: 007239/2011-40
CNPq: 47027/2012-0
Date 2014-07-01
Published in Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1840, n. 7, p. 2262-2271, 2014.
ISSN 0304-4165 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 2262-2271
Access rights Closed access
Type Article
Web of Science ID WOS:000338402400017

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