Sensitivity of Salmonella YG5161 for Detecting PAH-Associated Mutagenicity in Air Particulate Matter

Sensitivity of Salmonella YG5161 for Detecting PAH-Associated Mutagenicity in Air Particulate Matter

Author Umbuzeiro, Gisela A. Google Scholar
Kummrow, Fabio Autor UNIFESP Google Scholar
Morales, Daniel Alexandre Google Scholar
Alves, Debora Kristina M. Google Scholar
Lim, Hwanmi Google Scholar
Jarvis, Ian W. H. Google Scholar
Bergvall, Christoffer Google Scholar
Westerholm, Roger Google Scholar
Stenius, Ulla Google Scholar
Dreij, Kristian Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Stockholm Univ
Karolinska Inst
Abstract The Salmonella/microsome assay is the most used assay for the evaluation of air particulate matter (PM) mutagenicity and a positive correlation between strain TA98 responses and benzo[a]pyrene (B[a]P) levels in PM has been found. However, it seems that the major causes of PM mutagenicity in this assay are the nitro and oxy-PAHs. Salmonella YG5161, a 30-times more responsive strain to B[a]P has been developed. To verify if YG5161 strain was sufficiently sensitive to detect mutagenicity associated with B[a]P mutagenicity, PM samples were collected in Brazil and Sweden, extracted with toluene and tested in the Salmonella/microsome microsuspension assay. PAHs and B[a]P were determined and the extracts were tested with YG5161 and its parental strain TA1538. the extracts were also tested with YG1041 and its parental strain TA98. for sensitivity comparisons, we tested B[a]P and 1-nitropyrene (1-NP) using the same conditions. the minimal effective dose of B[a]P was 155 ng/plate for TA1538 and 7 ng/plate for YG5161. Although the maximum tested dose, 10 m(3)/plate containing 9 ng of B[a]P in the case of Brazilian sample, was sufficient to elicit a response in YG5161, mutagenicity was detected at a dose as low as 1 m(3)/plate (0.9 ng). This is probably caused by nitro-compounds that have been shown to be even more potent than B[a]P for YG5161. It seems that the mutagenicity of B[a]P present in PM is not detectable even with the use of YG5161 unless more efficient separation to remove the nitro-compounds from the PAH extract is performed. (C) 2014 Wiley Periodicals, Inc.
Keywords PAHs
salmonella/microsome
nitro-PAHs
mutagenicity
air pollution
Language English
Sponsor Swedish Research Council Formas
Cancer-och Allergi-fonden
Stockholm University
Date 2014-07-01
Published in Environmental and Molecular Mutagenesis. Hoboken: Wiley-Blackwell, v. 55, n. 6, p. 510-517, 2014.
ISSN 0893-6692 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 510-517
Origin http://dx.doi.org/10.1002/em.21861
Access rights Closed access
Type Article
Web of Science ID WOS:000339108600007
URI http://repositorio.unifesp.br/handle/11600/37917

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