Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii

Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii

Author Gegembauer, Gregory Autor UNIFESP Google Scholar
Araujo, Leticia Mendes Autor UNIFESP Google Scholar
Pereira, Edy Firmina Google Scholar
Rodrigues, Anderson Messias Autor UNIFESP Google Scholar
Paniago, Anamaria Mello Miranda Google Scholar
Hahn, Rosane Christine Google Scholar
Camargo, Zoilo Pires de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Mato Grosso do Sul (UFMS)
Univ Fed Mato Grosso UFMT
Abstract Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. in western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2012/06593-0
FAPESP: 2009/54024-2
Date 2014-07-01
Published in Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 8, n. 7, 12 p., 2014.
ISSN 1935-2735 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 12
Origin http://dx.doi.org/10.1371/journal.pntd.0002986
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000340551500035
URI http://repositorio.unifesp.br/handle/11600/37903

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