Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium

Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium

Autor Pereira, M. F. N. Autor UNIFESP Google Scholar
Fernandes, S. A. F. Autor UNIFESP Google Scholar
Nascimento, A. R. Autor UNIFESP Google Scholar
Siu, E. R. Autor UNIFESP Google Scholar
Hess, R. A. Google Scholar
Oliveira, C. A. Google Scholar
Porto, C. S. Autor UNIFESP Google Scholar
Lazari, M. F. M. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Illinois
Universidade Federal de Minas Gerais (UFMG)
Resumo The role of oestrogens in epididymal function is still unclear. Knockout of the oestrogen receptor ESR1 (Esr1-/-) or treatment with the anti-oestrogen Fulvestrant affect epididymal milieu and sperm motility. We investigated the effect of in vivo treatment of rats with Fulvestrant on: (i) expression of genes that may be important for the architecture and function of the epididymal epithelium: prominins 1 and 2, metalloproteinase 7, claudin 7, beta-catenin and cadherin 13, and (ii) levels of oestradiol and testosterone, and expression of oestrogen and androgen receptors, in the initial segment (IS), caput, corpus and cauda epididymis. Fulvestrant (i) reduced gene expression of prominin 1 (variant 1) in the caput, reduced prominin 1 protein content in the caput epididymis and in the efferent ductules, and increased the localization of prominin 1 in microvilli of the caput and corpus; (ii) reduced gene expression of prominin 2 in the corpus and cauda epididymis; (iii) increased the metalloproteinase 7 content in the apical region of principal cells from IS/caput; (iv) reduced in the corpus epididymis, but increased in the efferent ductules, the cadherin 13 mRNA level; (v) reduced testosterone but increased oestradiol levels in the corpus and cauda; (vi) increased the androgen receptor protein content in all regions of the epididymis, and the oestrogen receptor GPER in the corpus and cauda epididymis. in conclusion, treatment with Fulvestrant induced regional-specific changes in hormonal and steroid receptor content, and affected expression of proteins important for epithelial organization and absorption/secretion. the mechanisms of oestrogen action may differ among epididymal regions, which may contribute to determine region-specific sperm functions.
Assunto epididymis
oestrogens
gene expression
rat
animal models
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Número do financiamento FAPESP: 2008/56564-1
Data 2014-07-01
Publicado em Andrology. Hoboken: Wiley-Blackwell, v. 2, n. 4, p. 559-571, 2014.
ISSN 2047-2919 (Sherpa/Romeo, fator de impacto)
Editor Wiley-Blackwell
Extensão 559-571
Fonte http://dx.doi.org/10.1111/j.2047-2927.2014.00219.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000338038400010
URI http://repositorio.unifesp.br/handle/11600/37898

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