Preparation, Characterization, Cytotoxicity, and Genotoxicity Evaluations of Thiolated- and S-Nitrosated Superparamagnetic Iron Oxide Nanoparticles: Implications for Cancer Treatment

Preparation, Characterization, Cytotoxicity, and Genotoxicity Evaluations of Thiolated- and S-Nitrosated Superparamagnetic Iron Oxide Nanoparticles: Implications for Cancer Treatment

Author Seabra, Amedea Barozzi Autor UNIFESP Google Scholar
Pasquoto, Tatiane Google Scholar
Ferrarini, Ana Carolina Ferraz Autor UNIFESP Google Scholar
Santos, Marconi da Cruz Autor UNIFESP Google Scholar
Haddad, Paula S. Autor UNIFESP Google Scholar
Lima, Renata de Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de São Carlos (UFSCar)
Univ Sorocaba
Abstract Iron oxide magnetic nanoparticles have been proposed for an increasing number of biomedical applications, such as drug delivery. To this end, toxicological studies of their potent effects in biological media must be better evaluated. the aim of this study was to synthesize, characterize, and examine the potential in vitro cytotoxicity and genotoxicity of thiolated (SH) and S-nitrosated (S-NO) iron oxide superparamagnetic nanoparticles toward healthy and cancer cell lines. Fe3O4 nanoparticles were synthesized by coprecipitation techniques and coated with small thiol-containing molecules, such as mercaptosuccinic acid (MSA) or meso-2,3-dimercaptosuccinic acid (DMSA). the physical chemical, morphological, and magnetic properties of thiol-coating Fe3O4 nanoparticles were characterized by different techniques. the thiol groups on the surface of the nanoparticles were nitrosated, leading to the formation of S-nitroso-MSA- or S-nitroso-DMSA-Fe3O4 nanoparticles. the cytotoxicity and genotoxicity of thiolated and S-nitrosated nanoparticles were more deeply evaluated in healthy (3T3, human lymphocytes cells, and chinese hamster ovary cells) and cancer cell lines (MCF-7). the results demonstrated that thiol-coating iron oxide magnetic nanoparticles have few toxic effects in cells, whereas S-nitrosated-coated particles did cause toxic effects. Moreover, due to the superaramagnetic behavior of S-nitroso-Fe3O4 nanopartides, those particles can be guided to the target site upon the application of an external magnetic field, leading to local toxic effects in the tumor cells. Taken together, the results suggest the promise of S-nitroso-mgnetic nanopartides in cancer treatment.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2012/17053-7
FAPESP: 2011/10125-0
Date 2014-07-01
Published in Chemical Research in Toxicology. Washington: Amer Chemical Soc, v. 27, n. 7, p. 1207-1218, 2014.
ISSN 0893-228X (Sherpa/Romeo, impact factor)
Publisher Amer Chemical Soc
Extent 1207-1218
Origin http://dx.doi.org/10.1021/tx500113u
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000339462700013
URI http://repositorio.unifesp.br/handle/11600/37888

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