Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome

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dc.contributor.author Aitken, Elizabeth H.
dc.contributor.author Negri, Elnara M.
dc.contributor.author Barboza, Renato [UNIFESP]
dc.contributor.author Lima, Maria Ri
dc.contributor.author Alvarez, Jose M.
dc.contributor.author Marinho, Claudio R. F.
dc.contributor.author Caldini, Elia G.
dc.contributor.author Epiphanio, Sabrina
dc.date.accessioned 2016-01-24T14:37:27Z
dc.date.available 2016-01-24T14:37:27Z
dc.date.issued 2014-06-13
dc.identifier http://dx.doi.org/10.1186/1475-2875-13-230
dc.identifier.citation Malaria Journal. London: Biomed Central Ltd, v. 13, 10 p., 2014.
dc.identifier.issn 1475-2875
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37872
dc.description.abstract Background: the mechanisms through which infection with Plasmodium spp. result in lung disease are largely unknown. Recently a number of mouse models have been developed to research malaria-associated lung injury but no detailed ultrastructure studies of the disease in its terminal stages in a murine model have yet been published. the goal was to perform an ultrastructural analysis of the lungs of mice that died with malaria-associated acute lung injury/acute respiratory distress syndrome to better determine the relevancy of the murine models and investigate the mechanism of disease.Methods: DBA/2 mice were infected with Plasmodium berghei strain ANKA. Mice had their lungs removed immediately after death, processed using standard methods and viewed by transmission electron microscopy (TEM).Results: Infected red blood cell: endothelium contact, swollen endothelium with distended cytoplasmic extensions and thickening of endothelium basement membrane were observed. Septa were thick and filled with congested capillaries and leukocytes and the alveolar spaces contained blood cells, oedema and cell debris.Conclusion: Results show that the lung ultrastructure of P. berghei ANKA-infected mice has similar features to what has been described in post-mortem TEM studies of lungs from individuals infected with Plasmodium falciparum. These data support the use of murine models to study malaria-associated acute lung injury. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 10
dc.language.iso eng
dc.publisher Biomed Central Ltd
dc.relation.ispartof Malaria Journal
dc.rights Acesso aberto
dc.subject Malaria en
dc.subject Plasmodium en
dc.subject Acute lung injury (ALI) en
dc.subject Acute respiratory distress syndrome (ARDS) en
dc.subject Transmission electron microscopy en
dc.subject Murine model en
dc.title Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome en
dc.type Artigo
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Dept Immunol, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Med, Lab Med Invest 59, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Phys & Earth Sci, Diadema, Brazil
dc.description.affiliation Univ São Paulo, Dept Parasitol, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Phys & Earth Sci, Diadema, Brazil
dc.description.sponsorshipID FAPESP: 2009/53256-7
dc.description.sponsorshipID FAPESP: 2009/53889-0
dc.description.sponsorshipID CNPq: 306668/2012-2
dc.description.sponsorshipID FAPESP: 2011/195252-0
dc.identifier.file WOS000338889000001.pdf
dc.identifier.doi 10.1186/1475-2875-13-230
dc.description.source Web of Science
dc.identifier.wos WOS:000338889000001



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