Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome

Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome

Author Aitken, Elizabeth H. Google Scholar
Negri, Elnara M. Google Scholar
Barboza, Renato Autor UNIFESP Google Scholar
Lima, Maria Ri Google Scholar
Alvarez, Jose M. Google Scholar
Marinho, Claudio R. F. Google Scholar
Caldini, Elia G. Google Scholar
Epiphanio, Sabrina Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Background: the mechanisms through which infection with Plasmodium spp. result in lung disease are largely unknown. Recently a number of mouse models have been developed to research malaria-associated lung injury but no detailed ultrastructure studies of the disease in its terminal stages in a murine model have yet been published. the goal was to perform an ultrastructural analysis of the lungs of mice that died with malaria-associated acute lung injury/acute respiratory distress syndrome to better determine the relevancy of the murine models and investigate the mechanism of disease.Methods: DBA/2 mice were infected with Plasmodium berghei strain ANKA. Mice had their lungs removed immediately after death, processed using standard methods and viewed by transmission electron microscopy (TEM).Results: Infected red blood cell: endothelium contact, swollen endothelium with distended cytoplasmic extensions and thickening of endothelium basement membrane were observed. Septa were thick and filled with congested capillaries and leukocytes and the alveolar spaces contained blood cells, oedema and cell debris.Conclusion: Results show that the lung ultrastructure of P. berghei ANKA-infected mice has similar features to what has been described in post-mortem TEM studies of lungs from individuals infected with Plasmodium falciparum. These data support the use of murine models to study malaria-associated acute lung injury.
Keywords Malaria
Plasmodium
Acute lung injury (ALI)
Acute respiratory distress syndrome (ARDS)
Transmission electron microscopy
Murine model
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2009/53256-7
FAPESP: 2009/53889-0
CNPq: 306668/2012-2
FAPESP: 2011/195252-0
Date 2014-06-13
Published in Malaria Journal. London: Biomed Central Ltd, v. 13, 10 p., 2014.
ISSN 1475-2875 (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 10
Origin http://dx.doi.org/10.1186/1475-2875-13-230
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000338889000001
URI http://repositorio.unifesp.br/handle/11600/37872

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