Liposomes of phosphatidylcholine and cholesterol induce an M2-like macrophage phenotype reprogrammable to M1 pattern with the involvement of B-1 cells

Liposomes of phosphatidylcholine and cholesterol induce an M2-like macrophage phenotype reprogrammable to M1 pattern with the involvement of B-1 cells

Author Cruz-Leal, Yoelys Google Scholar
Lucatelli Laurindo, Maria Fernanda Autor UNIFESP Google Scholar
Osugui, Lika Autor UNIFESP Google Scholar
del Carmen Luzardo, Maria Google Scholar
Lopez-Requena, Alejandro Google Scholar
Eugenia Alonso, Maria Google Scholar
Alvarez, Carlos Google Scholar
Flavia Popi, Ana Autor UNIFESP Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Perez, Rolando Google Scholar
Eliana Lanio, Maria Google Scholar
Institution Univ Havana
Universidade Federal de São Paulo (UNIFESP)
Ctr Mol Immunol CIM
Abstract Macrophages respond to endogenous and non-self stimuli acquiring the M1 or M2 phenotypes, corresponding to classical or alternative activation, respectively. the role of B-1 cells in the regulation of macrophage polarization through the secretion of interleukin (IL)-10 has been demonstrated. However, the influence of B-1 cells on macrophage phenotype induction by an immunogen that suppress their ability to secrete IL-10 has not been explored. Here, we studied the peritoneal macrophage pattern induced by liposomes comprised of dipalmitoylphosphatidylcholine (DPPC) and cholesterol (Chol) carrying ovalbumin (OVA) (Lp DPPC/OVA), and the involvement of B-1 cells in macrophage polarization. Peritoneal cells from BALB/c, B-1 cells-deficient BALB/xid and C57BL/6 mice immunized with Lp DPPC/OVA and OVA in soluble form (PBS/OVA) were analyzed and stimulated or not in vitro with lipopolysaccharide (LPS). Peritoneal macrophages from BALB/c and C57BL/6 mice immunized with Lp DPPC/OVA showed an M2-like phenotype as evidenced by their high arginase activity without LPS stimulation. Upon stimulation, these macrophages were reprogrammable toward the M1 phenotype with the upregulation of nitric oxide (NO) and a decrease in IL-10 secretion. in addition, high IFN-gamma levels were detected in the culture supernatant of peritoneal cells from BALB/c and C57BL/6 mice immunized with Lp DPPC/OVA. Nevertheless, still high levels of arginase activity and undetectable levels of IL-12 were found, indicating that the switch to a classical activation state was not complete. in the peritoneal cells from liposomes-immunized BALB/xid mice, levels of arginase activity, NO, and IL-6 were below those from wild type animals, but the last two products were restored upon adoptive transfer of B-1 cells, together with an increase in IFN-gamma secretion. Summarizing, we have demonstrated that Lp DPPC/OVA induce an M2-like pattern in peritoneal macrophages reprogrammable to M1 phenotype after LPS stimulation, with the involvement of B-1 cells. (C) 2014 Elsevier GmbH. All rights reserved.
Keywords B-1 cells
Macrophage polarization
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
CIM, Cuba
Grant number CAPES: 111/11
Date 2014-06-01
Published in Immunobiology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 219, n. 6, p. 403-415, 2014.
ISSN 0171-2985 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 403-415
Access rights Closed access
Type Article
Web of Science ID WOS:000336017100001

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