The Kallikrein Inhibitor from Bauhinia bauhinioides (BbKI) shows antithrombotic properties in venous and arterial thrombosis models

The Kallikrein Inhibitor from Bauhinia bauhinioides (BbKI) shows antithrombotic properties in venous and arterial thrombosis models

Author Brito, Marlon V. Autor UNIFESP Google Scholar
Oliveira, Cleide de Autor UNIFESP Google Scholar
Salu, Bruno R. Autor UNIFESP Google Scholar
Andrade, Sonia A. Google Scholar
Malloy, Paula M. D. Autor UNIFESP Google Scholar
Sato, Ana C. Autor UNIFESP Google Scholar
Vicente, Cristina P. Google Scholar
Sampaio, Misako U. Autor UNIFESP Google Scholar
Maffei, Francisco H. A. Autor UNIFESP Google Scholar
Oliva, Maria Luiza V. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Abstract The Bauhinia bauhinioides Kallikrein Inhibitor (BbKI) is a Kunitz-type serine peptidase inhibitor of plant origin that has been shown to impair the viability of some tumor cells and to feature a potent inhibitory activity against human and rat plasma kallikrein (Kiapp 2.4 nmol/L and 5.2 nmol/L, respectively). This inhibitory activity is possibly responsible for an effect on hemostasis by prolonging activated partial thromboplastin time (aPTT). Because the association between cancer and thrombosis is well established, we evaluated the possible antithrombotic activity of this protein in venous and arterial thrombosis models. Vein thrombosis was studied in the vena cava ligature model in Wistar rats, and arterial thrombosis in the photochemical induced endothelium lesion model in the carotid artery of C57 black 6 mice. BbKI at a concentration of 2.0 mg/kg reduced the venous thrombus weight by 65% in treated rats in comparison to rats in the control group. the inhibitor prolonged the time for total artery occlusion in the carotid artery model mice indicating that this potent plasma kallikrein inhibitor prevented thrombosis. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Blood coagulation
Kallikrein
Kunitz inhibitor
Thrombosis
Trypsin inhibitor
Tumor cells
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2009/53766-5
FAPESP: 2012/23640-2
Date 2014-05-01
Published in Thrombosis Research. Oxford: Pergamon-Elsevier B.V., v. 133, n. 5, p. 945-951, 2014.
ISSN 0049-3848 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 945-951
Origin http://dx.doi.org/10.1016/j.thromres.2014.02.027
Access rights Closed access
Type Article
Web of Science ID WOS:000334739100038
URI http://repositorio.unifesp.br/handle/11600/37742

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