Evaluation of the methylation profile of exfoliated cell samples from patients with head and neck squamous cell carcinoma

Evaluation of the methylation profile of exfoliated cell samples from patients with head and neck squamous cell carcinoma

Author Bomfim Longo, Ana Luiza Autor UNIFESP Google Scholar
Rettori, Marianna M. Autor UNIFESP Google Scholar
Carvalho, Ana Carolina de Autor UNIFESP Google Scholar
Kowalski, Luiz Paulo Google Scholar
Carvalho, Andre Lopes Google Scholar
Vettore, Andre Luiz Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Hosp AC Camargo Fund Antonio Prudente
Hosp Canc Barretos
Abstract BackgroundSilencing of tumor suppressor genes plays a vital role in head and neck carcinogenesis. the purposes of this study were to determine the methylation profile of exfoliated tumors cells collected from patients with head and neck squamous cell carcinoma (HNSCC) and to evaluate its prognostic significance.MethodsThe methylation profile and level of a 20-gene panel were evaluated by quantitative methylation-specific polymerase chain reaction (qMSP) in exfoliated tumor cell samples from 96 patients with HNSCC.ResultsCCNA1 (60.4%), DCC (54.2%), and TIMP3 (35.4%) were frequently methylated in these samples. Patients with exfoliated tumors cells positive for DCC methylation showed a trend toward a lower local recurrence-free survival.ConclusionThese findings indicate that a low invasive method could be used to access the methylation profile of exfoliated cells from patients with HNSCC. Moreover, our data provide evidence that hypermethylation of DCC could be useful as prognostic indicator for this malignancy. (c) 2013 Wiley Periodicals, Inc. Head Neck 36: 631-637, 2014
Keywords qMSP
exfoliated cell
head and neck squamous cell carcinoma (HNSCC)
methylation
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2008/58460-9
FAPESP: 2005/02580-8
FAPESP: 07/56471-0
FAPESP: 07/56245-0
CNPq: 313181/2009-8
CNPq: 302360/2008-5
Date 2014-05-01
Published in Head and Neck-Journal for the Sciences and Specialties of the Head and Neck. Hoboken: Wiley-Blackwell, v. 36, n. 5, p. 631-637, 2014.
ISSN 1043-3074 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 631-637
Origin http://dx.doi.org/10.1002/hed.23345
Access rights Closed access
Type Article
Web of Science ID WOS:000334423500006
URI http://repositorio.unifesp.br/handle/11600/37707

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