Patterns of Gene Expression in Peripheral Blood Mononuclear Cells and Outcomes from Patients with Sepsis Secondary to Community Acquired Pneumonia

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dc.contributor.author Severino, Patricia
dc.contributor.author Silva, Eliezer
dc.contributor.author Baggio-Zappia, Giovana Lotici [UNIFESP]
dc.contributor.author Brunialti, Milena Karina Coló [UNIFESP]
dc.contributor.author Nucci, Laura Alejandra [UNIFESP]
dc.contributor.author Rigato, Otelo
dc.contributor.author Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
dc.contributor.author Machado, Flavia Ribeiro [UNIFESP]
dc.contributor.author Salomao, Reinaldo [UNIFESP]
dc.date.accessioned 2016-01-24T14:35:28Z
dc.date.available 2016-01-24T14:35:28Z
dc.date.issued 2014-03-25
dc.identifier http://dx.doi.org/10.1371/journal.pone.0091886
dc.identifier.citation Plos One. San Francisco: Public Library Science, v. 9, n. 3, 8 p., 2014.
dc.identifier.issn 1932-6203
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37557
dc.description.abstract Mechanisms governing the inflammatory response during sepsis have been shown to be complex, involving cross-talk between diverse signaling pathways. Current knowledge regarding the mechanisms underlying sepsis provides an incomplete picture of the syndrome, justifying additional efforts to understand this condition. Microarray-based expression profiling is a powerful approach for the investigation of complex clinical conditions such as sepsis. in this study, we investigate whole-genome expression profiles in mononuclear cells from survivors (n = 5) and non-survivors (n = 5) of sepsis. To circumvent the heterogeneity of septic patients, only patients admitted with sepsis caused by community-acquired pneumonia were included. Blood samples were collected at the time of sepsis diagnosis and seven days later to evaluate the role of biological processes or genes possibly involved in patient recovery. Principal Components Analysis (PCA) profiling discriminated between patients with early sepsis and healthy individuals. Genes with differential expression were grouped according to Gene Ontology, and most genes related to immune defense were up-regulated in septic patients. Additionally, PCA in the early stage was able to distinguish survivors from non-survivors. Differences in oxidative phosphorylation seem to be associated with clinical outcome because significant differences in the expression profile of genes related to mitochondrial electron transport chain (ETC) I-V were observed between survivors and non-survivors at the time of patient enrollment. Global gene expression profiles after seven days of sepsis progression seem to reproduce, to a certain extent, patterns collected at the time of diagnosis. Gene expression profiles comparing admission and follow-up samples differed between survivors and non-survivors, with decreased expression of genes related to immune functions in non-survivors. in conclusion, genes related to host defense and inflammatory response ontology were up-regulated during sepsis, consistent with the need for a host response to infection, and the sustainability of their expression in follow-up samples was associated with outcomes. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Albert Einstein Research and Education Institute - Hospital Israelita Albert Einstein
dc.format.extent 8
dc.language.iso eng
dc.publisher Public Library Science
dc.relation.ispartof Plos One
dc.rights Acesso aberto
dc.title Patterns of Gene Expression in Peripheral Blood Mononuclear Cells and Outcomes from Patients with Sepsis Secondary to Community Acquired Pneumonia en
dc.type Artigo
dc.contributor.institution Hosp Israelita Albert Einstein
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Hosp Sirio Libanes
dc.description.affiliation Hosp Israelita Albert Einstein, Inst Israelita Ensino & Pesquisa, Ctr Expt Res, São Paulo, Brazil
dc.description.affiliation Hosp Israelita Albert Einstein, Intens Care Unit, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo Unifesp, EPM, Hosp São Paulo, Div Infect Dis, São Paulo, Brazil
dc.description.affiliation Hosp Sirio Libanes, Intens Care Unit, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo Unifesp, EPM, Dept Gynecol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo Unifesp, Intens Care Unit, Hosp São Paulo, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo Unifesp, EPM, Hosp São Paulo, Div Infect Dis, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo Unifesp, EPM, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo Unifesp, Intens Care Unit, Hosp São Paulo, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: FAPESP 2006/58744-1
dc.identifier.file WOS000333675600014.pdf
dc.identifier.doi 10.1371/journal.pone.0091886
dc.description.source Web of Science
dc.identifier.wos WOS:000333675600014



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