Lysosomal integral membrane protein 2 (LIMP-2) restricts the invasion of Trypanosoma cruzi extracellular amastigotes through the activity of the lysosomal enzyme beta-glucocerebrosidase

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dc.contributor.author Goncalves, Viviane Martinelli [UNIFESP]
dc.contributor.author D'Almeida, Vania [UNIFESP]
dc.contributor.author Mueller, Karen Barbosa [UNIFESP]
dc.contributor.author Real, Fernando [UNIFESP]
dc.contributor.author Mortara, Renato Arruda [UNIFESP]
dc.date.accessioned 2016-01-24T14:35:23Z
dc.date.available 2016-01-24T14:35:23Z
dc.date.issued 2014-03-01
dc.identifier http://dx.doi.org/10.1016/j.micinf.2013.11.004
dc.identifier.citation Microbes and Infection. Amsterdam: Elsevier B.V., v. 16, n. 3, p. 253-260, 2014.
dc.identifier.issn 1286-4579
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37496
dc.description.abstract Lysosomal integral membrane protein 2 (LDM1P-2, SCARB2) is directly linked to beta-glucocerebrosidase enzyme (beta GC) and mediates the transport of this enzyme from the Golgi complex to lysosomes. Active beta GC cleaves the beta-glycosidic linkages of glucosylceramide, an intermediate in the metabolism of sphingoglycolipids, generating ceramide. in this study we used mouse embryonic fibroblasts (MEFs) deficient for LIMP-2 and observed that these cells were more susceptible to infection by extracellular amastigotes of the protozoan parasite Trypanosoma cruzi when compared to wild-type (WT) fibroblasts. the absence of LIMP-2 decreases the activity of beta GC measured in fibroblast extracts. Replacement of beta GC enzyme in LIMP-2 deficient fibroblasts restores the infectivity indices to those of WT cells in T cruzti invasion assays. Considering the participation of beta GC in the production of host cell ceramide, we propose that T cruzi extracellular amastigotes are more invasive to cells deficient in this membrane component. These results contribute to our understanding of the role of host cell lysosomal components in T cruzi invasion. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 253-260
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Microbes and Infection
dc.rights Acesso restrito
dc.subject LIMP-2 en
dc.subject beta-Glucocerebrosidase en
dc.subject Trypanosoma cruzi en
dc.subject Extracellular amastigotes en
dc.title Lysosomal integral membrane protein 2 (LIMP-2) restricts the invasion of Trypanosoma cruzi extracellular amastigotes through the activity of the lysosomal enzyme beta-glucocerebrosidase en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Psychobiol, BR-04023062 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Pediat, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Psychobiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Pediat, BR-04023062 São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 10/19335-4
dc.identifier.doi 10.1016/j.micinf.2013.11.004
dc.description.source Web of Science
dc.identifier.wos WOS:000347864600010



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