Effects of Allogeneic Bone Marrow Derived Mesenchymal Stromal Cell Therapy on Voiding Function in a Rat Model of Parkinson Disease

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dc.contributor.author Campeau, Lysanne
dc.contributor.author Soler, Roberto [UNIFESP]
dc.contributor.author Sittadjody, Sivanandane
dc.contributor.author Pareta, Rajesh
dc.contributor.author Nomiya, Masanori
dc.contributor.author Zarifpour, Mona
dc.contributor.author Opara, Emmanuel C.
dc.contributor.author Yoo, James J.
dc.contributor.author Andersson, Karl-Erik
dc.date.accessioned 2016-01-24T14:35:23Z
dc.date.available 2016-01-24T14:35:23Z
dc.date.issued 2014-03-01
dc.identifier http://dx.doi.org/10.1016/j.juro.2013.08.026
dc.identifier.citation Journal of Urology. New York: Elsevier B.V., v. 191, n. 3, p. 850-859, 2014.
dc.identifier.issn 0022-5347
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37493
dc.description.abstract Purpose: Cellular therapy induced transient urodynamic improvement in a rat model of Parkinson disease in which bladder dysfunction was noted after unilateral injection of 6-hydroxydopamine into the medial forebrain bundle. We sought to prolong the effect by injecting allogeneic rat bone marrow mesenchymal stromal cells before and after microencapsulation into the substantia nigra pars compacta.Materials and Methods: Female rats underwent unilateral stereotactic injection of 6-hydroxydopamine in the medial forebrain bundle. Injection was performed in the ipsilateral substantia nigra pars compacta using vehicle alone or vehicle with nonmicroencapsulated or microencapsulated rat bone marrow derived mesenchymal stromal cells. Rats were evaluated by cystometry 7, 14, 28 and 42 days after treatment. Brains were extracted for immunostaining.Results: At 42 days the nonmicroencapsulated group had lower threshold and intermicturition pressure, spontaneous activity and AUC than vehicle treated animals. Rats that received microencapsulated cells had lower threshold pressure at 28 days and lower spontaneous activity at 42 days than vehicle treated rats. Microencapsulated and nonmicroencapsulated rat bone marrow derived mesenchymal stromal cells were noted in the substantia nigra pars compacta up to 42 days after transplantation. At 42 days tyrosine hydroxylase positive neurons were more numerous in the substantia nigra pars compacta of the nonmicroencapsulated group, followed by the microencapsulated and vehicle treated groups.Conclusions: Urodynamic effects of the 6-hydroxydopamine lesion persisted up to 42 days after vehicle injection. Transplantation of nonmicroencapsulated rat bone marrow derived mesenchymal stromal cells improved urodynamic pressure by 42 days after treatment more markedly than microencapsulated cells. This was associated with more tyrosine hydroxylase positive neurons in the treated substantia nigra pars compacta of the nonmicroencapsulated group, suggesting that functional improvement requires a juxtacrine effect. en
dc.description.sponsorship American Urological Association Foundation
dc.description.sponsorship American Urological Association Southeastern Section Research Scholar Endowment Fund
dc.format.extent 850-859
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Journal of Urology
dc.rights Acesso restrito
dc.subject urinary bladder, neurogenic en
dc.subject Parkinson disease en
dc.subject mesenchymal stromal cells en
dc.subject urodynamics en
dc.subject substantia nigra en
dc.title Effects of Allogeneic Bone Marrow Derived Mesenchymal Stromal Cell Therapy on Voiding Function in a Rat Model of Parkinson Disease en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Wake Forest Univ
dc.contributor.institution NYU
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Fukushima Med Univ
dc.description.affiliation Wake Forest Univ, Bowman Gray Sch Med, Wake Forest Inst Regenerat Med, Winston Salem, NC USA
dc.description.affiliation NYU, Dept Urol, New York, NY USA
dc.description.affiliation Universidade Federal de São Paulo, Div Urol, São Paulo, Brazil
dc.description.affiliation Fukushima Med Univ, Dept Urol, Sch Med, Fukushima, Japan
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Div Urol, São Paulo, Brazil
dc.identifier.doi 10.1016/j.juro.2013.08.026
dc.description.source Web of Science
dc.identifier.wos WOS:000331123400096



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