New insights about cross-reactive epitopes of six trypanosomatid genera revealed that Crithidia and Leptomonas have antigenic similarity to L. (L.) chagasi

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dc.contributor.author Ferreira, Leandro Rodrigues
dc.contributor.author Kesper, Norival
dc.contributor.author Teixeira, Marta M. G.
dc.contributor.author Laurenti, Marcia Dalastra
dc.contributor.author Barbieri, C. L. [UNIFESP]
dc.contributor.author Lindoso, Jose Angelo
dc.contributor.author Umezawa, Eufrosina S.
dc.date.accessioned 2016-01-24T14:35:22Z
dc.date.available 2016-01-24T14:35:22Z
dc.date.issued 2014-03-01
dc.identifier http://dx.doi.org/10.1016/j.actatropica.2013.11.010
dc.identifier.citation Acta Tropica. Amsterdam: Elsevier B.V., v. 131, p. 41-46, 2014.
dc.identifier.issn 0001-706X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37483
dc.description.abstract We investigated whether ELISA using crude antigens from insect and plant trypanosomatids, which are non-pathogenic and easily cultivated in large scale, has the same positivity data as Leishmania (Leishmania) chagasi, the etiological agent of human visceral leishmaniasis (VL) or canine leishmaniasis (CanL), or as Trypanosoma cruzi, the etiological agent of Chagas disease (CD). the antigens from Crithidia fasciculata, Crithidia luciliae, and Leptomonas seymouri showed 100% cross-reactivity with VL and CanL samples, with no statistically titers differences from L. (L.) chagasi, however, 34% (17/50) of VL samples revealed higher titers using the insect trypanosomatids than the homologous antigen. On the other hand, antigens from Strigomonas culicis, Angomonas deanei, and Phytomonas serpens showed low cross-reactivity with VL and CanL samples. the sera from patients with American tegumentary leishmaniasis showed low levels of cross-reactivity with all trypanosomatids investigated, even with L. (L) chagasi, without titers dissimilarity among them. These parasites were also worthless as antigen source for detection of CD cases, which required homologous antigens to reach 100% positivity. This study showed, by ELISA, that crude extract of Crithidia and Leptomonas have epitopes similar to L. (L.) chagasi, which supports the idea of using them as antigens source for the serodiagnosis of visceral leishmaniasis. Published by Elsevier B.V. en
dc.description.sponsorship LIM49-FMUSP
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 41-46
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Acta Tropica
dc.rights Acesso restrito
dc.subject L. (L.) chagasi en
dc.subject Trypanosoma cruzi en
dc.subject Crithidia en
dc.subject Leptomonas en
dc.subject Trypanosomatids en
dc.subject Cross-reactivity en
dc.title New insights about cross-reactive epitopes of six trypanosomatid genera revealed that Crithidia and Leptomonas have antigenic similarity to L. (L.) chagasi en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Inst Infectol Emilio Ribas São Paulo
dc.description.affiliation Univ São Paulo, Inst Med Trop Salo Paulo, BR-09500900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Med, Dept Prevent Med, BR-09500900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-09500900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Med, Dept Patol, BR-09500900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliation Inst Infectol Emilio Ribas São Paulo, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, São Paulo, Brazil
dc.identifier.doi 10.1016/j.actatropica.2013.11.010
dc.description.source Web of Science
dc.identifier.wos WOS:000332905500007



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