Decreased expression of proteins involved in energy metabolism in the hippocampal granular layer of rats submitted to the pilocarpine epilepsy model

Decreased expression of proteins involved in energy metabolism in the hippocampal granular layer of rats submitted to the pilocarpine epilepsy model

Author Araujo, Bruno Autor UNIFESP Google Scholar
Torres, Laila Autor UNIFESP Google Scholar
Stein, Mariana Autor UNIFESP Google Scholar
Cabral, Francisco Romero Google Scholar
Herai, Roberto Google Scholar
Okamoto, Oswaldo Google Scholar
Cavalheiro, Esper Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Hosp Israelita Albert Einstein
Fac Ciencias Med Santa Casa São Paulo
Univ Calif San Diego
Universidade de São Paulo (USP)
Abstract Long-term structural and functional changes in the hippocampus have been identified as the primary physiopathological basis for temporal lobe epilepsy. These changes include reactive gliosis and granule cell axonal sprouting within the dentate gyrus. the intimate mechanisms of these changes are beginning to be revealed. Here, we show the possibility of using laser capture microdissection (LCM) to isolate the dentate granular cell layer of Wistar rats submitted to the pilocarpine model of epilepsy. Using two-dimensional gel electrophoresis (2-D PAGE) and mass spectrometry for laser-captured cells, we identified molecular events that could be altered as part of the epileptic pathogenic process. According to our results, eight proteins related to energy metabolism were differentially expressed between both the control and pilocarpine-treated animals. These results provide, for the first time, new molecular insights into the altered protein profile of the epileptogenic dentate gyrus and can contribute to a better understanding of the phenomena involved in the genesis and maintenance of the epileptic state. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
Keywords Energy metabolism
Temporal lobe epilepsy
Laser capture microdissection
Two-dimensional gel electrophoresis
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
CInAPCe (Brazil)
Grant number FAPESP: 09/05764-3
Date 2014-02-21
Published in Neuroscience Letters. Clare: Elsevier B.V., v. 561, p. 46-51, 2014.
ISSN 0304-3940 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 46-51
Origin http://dx.doi.org/10.1016/j.neulet.2013.12.040
Access rights Closed access
Type Article
Web of Science ID WOS:000331858100009
URI http://repositorio.unifesp.br/handle/11600/37444

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