Multilocus sequence typing scheme for the Mycobacterium abscessus complex

Multilocus sequence typing scheme for the Mycobacterium abscessus complex

Author Macheras, Edouard Google Scholar
Konjek, Julie Google Scholar
Roux, Anne-Laure Google Scholar
Thiberge, Jean-Michel Google Scholar
Bastian, Sylvaine Google Scholar
Leao, Sylvia Cardoso Autor UNIFESP Google Scholar
Palaci, Moises Google Scholar
Sivadon-Tardy, Valerie Google Scholar
Gutierrez, Cristina Google Scholar
Richter, Elvira Google Scholar
Ruesch-Gerdes, Sabine Google Scholar
Pfyffer, Gaby E. Google Scholar
Bodmer, Thomas Google Scholar
Jarlier, Vincent Google Scholar
Cambau, Emmanuelle Google Scholar
Brisse, Sylvain Google Scholar
Caro, Valerie Google Scholar
Rastogi, Nalin Google Scholar
Gaillard, Jean-Louis Google Scholar
Heym, Beate Google Scholar
Institution Hop Ambroise Pare
Hop Raymond Poincard
Univ Versailles St Quentin Yvelines
Inst Pasteur
Ctr Natl Reference Mycobacteries & Resistance Myc
Grp Hosp Pitie Salpetriere
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Espirito Santo
Caribbean Publ Hlth Agcy CARPHA
Forschungszentrum Borstel
Zentrum LaborMed
Univ Bern
Grp Hosp Lariboisiere Fernand Widal
Abstract We developed a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus sensu lato, based on the partial sequencing of seven housekeeping genes: argH, cya, glpK, gnd, murC, pta and purH. This scheme was used to characterize a collection of 227 isolates recovered between 1994 and 2010 in France, Germany, Switzerland and Brazil. We identified 100 different sequence types (STs), which were distributed into three groups on the tree obtained by concatenating the sequences of the seven housekeeping gene fragments (3576 bp): the M. abscessus sensu stricto group (44 STs), the M. massiliense group (31 STs) and the M. bolletii group (25 STs). SplitTree analysis showed a degree of intergroup lateral transfers. There was also evidence of lateral transfer events involving rpoB. the most prevalent STs in our collection were ST1 (CC5; 20 isolates) and ST23 (CC3; 31 isolates). Both STs were found in Europe and Brazil, and the latter was implicated in a large post-surgical procedure outbreak in Brazil. Respiratory isolates from patients with cystic fibrosis belonged to a large variety of STs; however, ST2 was predominant in this group of patients.Our MLST scheme, publicly available at, offers investigators a valuable typing tool for M. abscessus sensu lato in future epidemiological studies throughout the world. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Keywords Mycobacterium abscessus complex
Molecular typing
Multilocus sequence typing
Phylogenetic analysis
Molecular identification
Cystic fibrosis
Opportunistic mycobacteria
Non-tuberculous mycobacteria
Language English
Sponsor Vaincre la Mucoviscidose
Date 2014-02-01
Published in Research in Microbiology. Amsterdam: Elsevier B.V., v. 165, n. 2, p. 82-90, 2014.
ISSN 0923-2508 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 82-90
Access rights Closed access
Type Article
Web of Science ID WOS:000347590900003

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