ORC1/CDC6 and MCM7 distinct associate with chromatin through Trypanosoma cruzi life cycle

ORC1/CDC6 and MCM7 distinct associate with chromatin through Trypanosoma cruzi life cycle

Author Calderano, Simone Google Scholar
Godoy, Patricia Google Scholar
Soares, Daiane Google Scholar
Sant'Anna, Osvaldo Augusto Google Scholar
Schenkman, Sergio Autor UNIFESP Google Scholar
Elias, M. Carolina Google Scholar
Institution Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Abstract Trypanosoma cruzi alternates between replicative and non-replicative stages. We analyzed the expression of components of the pre-replication machinery TcORC1/CDC6 and TcMCM7 and their interaction with DNA in all T. cruzi stages. TcORC1/CDC6 remains in the nuclear space during all stages of the life cycle and interacts with DNA in the replicative stages; however, it does not bind to DNA in the non-replicative forms. Moreover, TcMCM7 is not present in the non-replicative stages. These data suggest that the lacking of DNA replication during the T. cruzi life cycle may be a consequence of the blocking of TcORC1/CDC6-DNA interaction and of the down regulation of the TcMCM7 expression. (C) 2014 Elsevier B.V. All rights reserved.
Keywords DNA replication
ORC1/CDC6
MCM7
Trypanosoma cruzi
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2009/53693-8
FAPESP: 2013/07467-1
Date 2014-02-01
Published in Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 193, n. 2, p. 110-113, 2014.
ISSN 0166-6851 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 110-113
Origin http://dx.doi.org/10.1016/j.molbiopara.2014.03.004
Access rights Closed access
Type Article
Web of Science ID WOS:000336115400006
URI http://repositorio.unifesp.br/handle/11600/37380

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