Association of COX2 gene hypomethylation with intestinal type gastric cancer in samples of patients from northern Brazil

Association of COX2 gene hypomethylation with intestinal type gastric cancer in samples of patients from northern Brazil

Author Vieira de Melo, Cynthia Farias Google Scholar
Gigek, Carolina Oliveira Autor UNIFESP Google Scholar
Silva, Juarez Nobrega da Google Scholar
Cardoso Smith, Marilia de Arruda Autor UNIFESP Google Scholar
Araujo, Rubistenia Miranda de Google Scholar
Burbano, Rommel Rodriguez Google Scholar
Lima, Eleonidas Moura Google Scholar
Institution Univ Fed Paraiba
Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Abstract To verify the methylation status of THBS1, GPX3, and COX2 genes and to evaluate their association with Helicobacter pylori in gastric adenocarcinomas. Methylation-sensitive restriction enzyme PCR assay was performed in 16 diffuse type gastric cancer samples, 23 intestinal type, and 15 normal stomach tissue. the presence of H. pylori was performed by amplification of the fragment of the 16S rRNA. Statistical analyses were performed using Fisher's exact test. the hypermethylation of GPX3, THBS1, and COX2 occurred in 18 (n = 7), 5 (n = 2), and 36 % (n = 14) of gastric cancer samples, respectively, whereas in normal samples, it was found in 13, 7, and 67 %. the presence of H. pylori was detected in 67 % of gastric cancer samples and 67 % in normal gastric samples. the methylation of THBS1 and GPX3 was not significantly different between the types of tumors, normal sample, the presence of H. pylori, or clinicopathological variables studied (P > 0.05). However, the methylation status of the gene COX2 is significantly different between normal tissue and intestinal type gastric cancer (P = 0.02). Therefore, our results suggest that the methylation status of the gene COX2 is associated with the intestinal type of gastric cancer.
Keywords Gastric cancer
Methylation
COX2
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2014-02-01
Published in Tumor Biology. Dordrecht: Springer, v. 35, n. 2, p. 1107-1111, 2014.
ISSN 1010-4283 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1107-1111
Origin http://dx.doi.org/10.1007/s13277-013-1148-6
Access rights Closed access
Type Article
Web of Science ID WOS:000332026300032
URI http://repositorio.unifesp.br/handle/11600/37367

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